Khan A M, Gupta S, Katiyar J C, Srivastava V K
Parasitology Division, Central Drug Research Institute, Lucknow, India.
Indian J Exp Biol. 1996 Oct;34(10):1015-8.
Correlation between the degree of protection and induced serum antibody response in hamsters immunized with somatic and excretory-secretory (ES) antigens of adult. A. ceylanicum was investigated. Hamsters were immunized with non purified and Sephadex G-200 fractions (F1, F2 and F3) of somatic and ES antigens. The degree of protection was assessed in terms of percent worm reduction compared to controls against challenge infection. Induced humoral antibody response was determined by ELISA. Both somatic and ES antigens had shown moderate to significant protection but the latter was found more immunogenic as highest level of protection (67.02%) was achieved by these antigens. Humoral antibody was found highly elevated in animals immunized with protective doses of somatic and ES antigens. The maximum serum antibody titer i.e. 1:3200 was noticed in animals immunized with high protective dose (64.59% protection) of fraction F1 of ES antigens. Antibody titer correspond to the degree of protection and a positive correlation between induced humoral antibodies and protection level was established.
用成年锡兰钩虫的体抗原和排泄分泌（ES）抗原免疫仓鼠后，其保护程度与诱导的血清抗体反应之间的相关性进行了研究。仓鼠用体抗原和ES抗原的非纯化及葡聚糖凝胶G - 200组分（F1、F2和F3）进行免疫。保护程度根据与对照相比针对攻击感染的蠕虫减少百分比来评估。通过酶联免疫吸附测定法（ELISA）测定诱导的体液抗体反应。体抗原和ES抗原均显示出中度至显著的保护作用，但发现后者免疫原性更强，因为这些抗原实现了最高水平的保护（67.02%）。在用体抗原和ES抗原的保护剂量免疫的动物中发现体液抗体高度升高。在用ES抗原F1组分的高保护剂量（64.59%保护）免疫的动物中观察到最高血清抗体滴度，即1:3200。抗体滴度与保护程度相对应，并且在诱导的体液抗体与保护水平之间建立了正相关。
