Moeljono M P, Thatcher W W, Bazer F W, Frank M, Owens L J, Wilcox C J
Prostaglandins. 1977 Sep;14(3):543-55. doi: 10.1016/0090-6980(77)90268-4.
Polyvinyl catheters were inserted into the right and left utero-ovarian veins (UOV) and saphenous vein (SV) and artery (SA) of six non-pregnant (O) and five pregnant (P) gilts on day 11 after onset estrus. Beginning on day 12, UOV blood samples were collected at 15-min intervals from 0800 to 1100 hr and 2000 to 2300 hr, and single samples were taken at 1200 and 2400 hrs. Peripheral blood (SA or SV) was sampled at 0800, 1200, 2000 and 2400 hr until gilts returned to estrus (X = 20.6 days) or day 24 of pregnancy. UOV plasma PGF concentrations (ng/ml; n = 1929) were measured by RIA. Status (P vs O) by day interactions were detected (P less than .01) but variances among treatments were heterogenous (P less than .01). Curvilinear day trends were detected for PGF in 0 gilts (P less than .01) but not P gilts. PGF peaks, defined as concentrations greater than two SD above the mean concentration for each gilt, occurred with greater frequency (chi2 = 16.4; P less than .01) in O than P gilts; and mean peak levels (X +/- SE) were 5.04 +/- .27 and 3.84 +/- .13 ng/ml, respectively. Progesterone concentrations were maintained in pregnant pigs and were indicative of luteal maintenance. Systematic differences in day trends of utero-ovarian venous plasma estradiol were detected between O and P pigs. These differences may be of paramount physiological importance and are discussed.
在发情开始后的第11天，将聚乙烯导管插入6头未怀孕（O组）和5头怀孕（P组）后备母猪的左右子宫卵巢静脉（UOV）以及隐静脉（SV）和动脉（SA）。从第12天开始，在08:00至11:00以及20:00至23:00每隔15分钟采集一次UOV血样，并在12:00和24:00采集单个样本。在08:00、12:00、20:00和24:00采集外周血（SA或SV）样本，直至后备母猪恢复发情（X = 20.6天）或怀孕第24天。通过放射免疫分析法（RIA）测定UOV血浆中前列腺素F（PGF）的浓度（ng/ml；n = 1929）。检测到状态（P组与O组）与天数之间的交互作用（P < 0.01），但各处理之间的方差不齐（P < 0.01）。在O组后备母猪中检测到PGF呈曲线形的天数趋势（P < 0.01），但在P组中未检测到。PGF峰值定义为高于每头后备母猪平均浓度两个标准差以上的浓度，其出现频率在O组后备母猪中高于P组（χ2 = 16.4；P < 0.01）；平均峰值水平（X ± SE）分别为5.04 ± 0.27和3.84 ± 0.13 ng/ml。怀孕母猪的孕酮浓度保持稳定，表明黄体功能维持正常。检测到O组和P组母猪子宫卵巢静脉血浆雌二醇的天数趋势存在系统性差异。这些差异可能具有至关重要的生理意义，将予以讨论。
