Puurunen K, Sirviö J, Koistinaho J, Miettinen R, Haapalinna A, Riekkinen P, Sivenius J
A.I. Virtanen Institute, University of Kuopio, Finland.
Stroke. 1997 Mar;28(3):623-31. doi: 10.1161/01.str.28.3.623.
The purpose of this study was to determine whether an enriched housing environment and/or systemic administration of the alpha2-adrenergic receptor antagonist atipamezole facilitate the rate of spatial learning after global ischemia in rats.
Carotid arteries were closed for 20 minutes after permanent cauterization of vertebral arteries on the previous day. Enriched-environment housing and drug/saline treatment were begun 3 days after ischemia. For rehabilitation, housing in an enriched environment was combined with exploration in a labyrinth. Behavioral tests (the open-arena test and water-maze learning set task) were performed after 1-week periods of drug/saline treatment three times. In addition, the open-arena test was performed to evaluate the baseline level of animals 2 days after the induction of ischemia and at the end of the experiment, when the water-maze task was assessed in another room.
Rats housed in an enriched environment after ischemia showed better acquisition of the water-maze learning set task after 1 week of housing. The influence of atipamezole treatment on this parameter did not reach statistical significance. In the open-arena test, ischemic animals were slightly hyperactive; however, this symptom was eliminated by housing in an enriched environment.
The present data suggest that housing in an enriched environment facilitates the rate of spatial learning in rats with global ischemia. Rehabilitation also alleviated the hyperactivity observed in ischemic animals.
本研究旨在确定丰富的饲养环境和/或全身给予α2-肾上腺素能受体拮抗剂阿替美唑是否能促进大鼠全脑缺血后的空间学习速度。
在前一天永久性烧灼椎动脉后，将颈动脉闭塞20分钟。缺血3天后开始丰富环境饲养和药物/生理盐水治疗。为进行康复训练，将丰富环境饲养与迷宫探索相结合。在药物/生理盐水治疗1周后，分三次进行行为测试（旷场试验和水迷宫学习集任务）。此外，在缺血诱导后2天以及实验结束时（此时在另一个房间评估水迷宫任务）进行旷场试验，以评估动物的基线水平。
缺血后饲养在丰富环境中的大鼠在饲养1周后水迷宫学习集任务的习得情况更好。阿替美唑治疗对该参数的影响未达到统计学意义。在旷场试验中，缺血动物略显多动；然而，通过饲养在丰富环境中，这种症状得以消除。
目前的数据表明，丰富环境饲养可促进全脑缺血大鼠的空间学习速度。康复训练还减轻了缺血动物中观察到的多动症状。
