Maier C E, Miller R H
Dept. of Anatomy, School of Medicine, Case Western Reserve University, Cleveland, OH 44106-4930, USA.
J Neurosci Res. 1997 Feb 15;47(4):361-71. doi: 10.1002/(sici)1097-4547(19970215)47:4<361::aid-jnr1>3.0.co;2-c.
Oligodendrocyte precursors originate in the ventral ventricular zone of the developing spinal cord. To examine whether the notochord is essential for the development of oligodendrocytes in Xenopus spinal cord the notochord was prevented from forming, ablated, or transplanted during early stages of development. Differentiated oligodendrocytes did not appear in spinal cord regions lacking a notochord in animals in which notochord failed to develop after UV irradiation at the one-cell stage. Similarly, differentiated oligodendrocytes were not detected in the spinal cord adjacent to the site of segmental notochord ablation at embryonic or larval stages. Transplantation of an additional notochord dorsal to the spinal cord induced the premature appearance of differentiated oligodendrocytes in adjacent lateral and dorsal spinal cord white matter. These results indicate that the development of Xenopus spinal cord oligodendrocytes is dependent on local influences from the notochord and suggest that the notochord is essential for oligodendrocyte development in Xenopus spinal cord.
少突胶质细胞前体起源于发育中的脊髓腹侧脑室区。为了研究脊索对于非洲爪蟾脊髓中少突胶质细胞发育是否至关重要，在发育早期对脊索进行了阻止其形成、切除或移植的操作。在单细胞期经紫外线照射后脊索未能发育的动物中，缺乏脊索的脊髓区域未出现分化的少突胶质细胞。同样，在胚胎期或幼体期节段性脊索切除部位相邻的脊髓中也未检测到分化的少突胶质细胞。在脊髓背侧移植额外的脊索会诱导相邻的脊髓侧方和背侧白质中过早出现分化的少突胶质细胞。这些结果表明，非洲爪蟾脊髓少突胶质细胞的发育依赖于来自脊索的局部影响，并提示脊索对于非洲爪蟾脊髓中少突胶质细胞的发育至关重要。
