Chen D Q, Purcell A H
Department of Environmental Science, Policy and Management, University of California, Berkeley, CA 94720-3112, USA.
Curr Microbiol. 1997 Apr;34(4):220-5. doi: 10.1007/s002849900172.
The occurrence of a secondary bacterial symbiont (PASS) of pea aphid, Acyrthosiphon pisum (Harris), was detected by polymerase chain reaction (PCR) with specific nucleotide primers based on PASS 16S rDNA nucleotide sequences from over 80% (50/57) of clones of pea aphid collected from widely separated locations in California. PASS was also detected by PCR in both red and green phenotypes of rose aphid,Macrosiphum rosae (L.), but not in six other species of aphids examined, including blue alfalfa aphid (A. kondoi Shinji). The nucleotide sequences of the PCR-amplified, partial 16S rDNAs (1060 bp) from pea aphid and rose aphid were identical and 99.9% similar to the published 16S rDNA of PASS. PASS and a recently described new rickettsia of pea aphid (PAR) were transmitted by needle injection of hemolymph from positive pea aphid clones into negative clones and into blue alfalfa aphids. Both PASS and PAR were maintained in the offspring of some of the injected mother aphids via high rate of maternal transmission.
基于来自加利福尼亚州广泛分布地点采集的超过80%（50/57）的豌豆蚜克隆体的次生细菌共生体（PASS）16S rDNA核苷酸序列，通过聚合酶链反应（PCR）和特异性核苷酸引物检测到豌豆蚜（豆无网长管蚜，哈里斯）中存在次生细菌共生体。通过PCR在蔷薇长管蚜（蔷薇长管蚜，林奈）的红色和绿色表型中也检测到了PASS，但在所检测的其他六种蚜虫中未检测到，包括蓝苜蓿蚜（日本无网长管蚜）。从豌豆蚜和蔷薇长管蚜中PCR扩增得到的部分16S rDNA（1060 bp）的核苷酸序列相同，且与已发表的PASS的16S rDNA相似度为99.9%。通过将来自阳性豌豆蚜克隆体的血淋巴经针刺注射到阴性克隆体和蓝苜蓿蚜中，实现了PASS和最近描述的豌豆蚜新立克次氏体（PAR）的传播。PASS和PAR在一些注射过的母蚜后代中通过高比例的母系传播得以维持。
