Purdy C W, Raleigh R H, Collins J K, Watts J L, Straus D C
USDA Agricultural Research Service, Conservation and Production Research Laboratory, Bushland, TX 79012, USA.
Curr Microbiol. 1997 Apr;34(4):244-9. doi: 10.1007/s002849900177.
Ninety-one isolates of Pasteurella multocida (Pm)and 124 of Pasteurella haemolytica (Ph) were recovered from the lungs of calves that died of bovine respiratory tract disease (BRTD). Nine Pm enzyme profiles (A through I) and 9 Ph enzyme profiles (J through R) were determined for the Pasteurella isolates. The Pm isolates were relatively evenly divided among the enzyme profiles, with one exception, profile I. The Ph isolates were not evenly distributed among the profiles. Fifty of the 91 Pm isolates were serotyped. Forty-two Pm isolates were positive for capsule type A, and 8 were untypable. Five somatic type antigen profiles (3; 3,4; 3,7; 3,4,7; and 4) were identified among the 50 serotyped Pm isolates; one isolate was untypable. The Ph isolates were further divided through serotyping and grouped as follows: 74 (60%) Pasteurella haemolytica A1 (PhA1), 12 (10%) PhA2, 4 (3%) PhA5, and 34(27%) PhA6. Eighty-one percent of the Ph serotypes were clustered in the M and N enzyme profile. The P enzyme profile was almost unique to PhA2 (8 of 12, 67% of PhA2 isolates). Results of this study indicate a need to collect more data on Ph serotypes at the state veterinary diagnostic laboratories.
从死于牛呼吸道疾病（BRTD）的犊牛肺中分离出91株多杀性巴氏杆菌（Pm）和124株溶血巴氏杆菌（Ph）。对这些巴氏杆菌分离株确定了9种Pm酶谱（A至I）和9种Ph酶谱（J至R）。Pm分离株在酶谱中分布相对均匀，但谱I除外。Ph分离株在各谱中分布不均。对91株Pm分离株中的50株进行了血清分型。42株Pm分离株A型荚膜呈阳性，8株无法分型。在50株血清分型的Pm分离株中鉴定出5种菌体抗原谱（3；3,4；3,7；3,4,7；和4）；1株无法分型。Ph分离株通过血清分型进一步划分并分组如下：74株（60%）溶血巴氏杆菌A1型（PhA1），12株（10%）PhA2型，4株（3%）PhA5型，34株（27%）PhA6型。81%的Ph血清型聚集在M和N酶谱中。P酶谱几乎是PhA2型所特有的（12株中的8株，占PhA2分离株的67%）。本研究结果表明，需要在州兽医诊断实验室收集更多关于Ph血清型的数据。
