Majumdar D, Wyche J H
Department of Biology, Norfolk State University, Norfolk, VA 23504, USA.
Curr Microbiol. 1997 Apr;34(4):258-63. doi: 10.1007/s002849900179.
We previously reported the DNA sequence and expression of the Chlorobium vibrioforme glutamyl-tRNA reductase (hemA) gene (Majumdar et al., Arch Microbiol 156:281, 1991). The sequence downstream of the hemA gene indicated homology to Escherichia coli and Bacillus subtilis porphobilinogen deaminase (hemC) gene. The Chlorobium gene was confirmed to be the porphobilinogen deaminase gene, and complete sequence of the structural gene was obtained. A 2.8-kb DNA fragment containing the 1.3-kb hemA gene of Chlorobium was cloned into a hemC auxotroph (Sz16) of Bacillus subtilis, and complementation of the auxotroph to prototrophy was achieved. DNA sequence data showed a single open reading frame of 840 bp coding a protein of 279 amino acid residues. The deduced amino acid sequence of the Chlorobium porphobilinogen deaminase revealed 39% to 46% homology with the corresponding prokaryotic and eukaryotic sequences.
我们之前报道了绿弯菌谷氨酸-tRNA还原酶（hemA）基因的DNA序列及表达情况（马宗达等人，《微生物学档案》156:281，1991年）。hemA基因下游的序列显示与大肠杆菌和枯草芽孢杆菌的胆色素原脱氨酶（hemC）基因具有同源性。绿弯菌的该基因被确认为胆色素原脱氨酶基因，并获得了结构基因的完整序列。将包含1.3 kb绿弯菌hemA基因的2.8 kb DNA片段克隆到枯草芽孢杆菌的hemC营养缺陷型菌株（Sz16）中，实现了该营养缺陷型菌株向原养型的互补。DNA序列数据显示有一个840 bp的单一开放阅读框，编码一个由279个氨基酸残基组成的蛋白质。绿弯菌胆色素原脱氨酶的推导氨基酸序列与相应的原核和真核序列显示出39%至46%的同源性。
