编码大肠杆菌K12胆色素原脱氨酶的hemC基因座的核苷酸序列。
Nucleotide sequence of the hemC locus encoding porphobilinogen deaminase of Escherichia coli K12.
作者信息
Thomas S D, Jordan P M
出版信息
Nucleic Acids Res. 1986 Aug 11;14(15):6215-26. doi: 10.1093/nar/14.15.6215.
Abstract
Porphobilinogen deaminase, the product of the hemC locus in Escherichia coli K12, catalyses the tetrapolymerisation of porphobilinogen (PBG) into the hydroxymethylbilane, preuroporphyrinogen. The hemC locus has been subcloned from the Clarke and Carbon plasmid pLC41-4. The sequence of the hemC structural gene and flanking DNA was determined by the dideoxy chain termination method of Sanger. The structural gene for hemC is located within a 942bp sequence encoding the monomeric PBG deaminase, molecular weight 33,857. The extent of the coding region was confirmed by sequencing the N-terminus of the purified enzyme and by determination of the molecular weight. The hemC locus is closely linked to the cyaA locus, the genes being transcribed in a divergent manner. Upstream of the hemC coding region, a possible promoter and three repeated GGATG sequences were identified. This is the first report of a complete DNA sequence for a structural gene specifying an enzyme of the heme biosynthetic pathway in prokaryotes.
摘要
胆色素原脱氨酶是大肠杆菌K12中hemC基因座的产物，它催化胆色素原（PBG）四聚化形成羟甲基胆素，即尿卟啉原。hemC基因座已从克拉克和卡本质粒pLC41 - 4中进行亚克隆。hemC结构基因及侧翼DNA的序列通过桑格双脱氧链终止法测定。hemC的结构基因位于一段942bp的序列内，该序列编码单体PBG脱氨酶，分子量为33,857。通过对纯化酶的N端进行测序以及测定分子量，证实了编码区的范围。hemC基因座与cyaA基因座紧密相连，两个基因以相反方向转录。在hemC编码区上游，鉴定出一个可能的启动子和三个重复的GGATG序列。这是关于原核生物中指定血红素生物合成途径中一种酶的结构基因完整DNA序列的首次报道。
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