新生儿血培养采血量与凝固酶阴性葡萄球菌败血症的检测
Blood culture volume and detection of coagulase negative staphylococcal septicaemia in neonates.
作者信息
Jawaheer G, Neal T J, Shaw N J
机构信息
Neonatal Intensive Care Unit, Fazakerley Hospital, Liverpool.
出版信息
Arch Dis Child Fetal Neonatal Ed. 1997 Jan;76(1):F57-8. doi: 10.1136/fn.76.1.f57.
Abstract
A prospective, blind study was carried out to determine: the amount of blood submitted for culture from neonates; whether small blood volumes resulted in false negative results; and whether there was a temporal relation between volume of blood cultured and time to positivity. Seventy three bottles were evaluated. They contained a median of 0.63 ml of blood. Twenty nine bottles (39.7%) contained less than 0.5 ml of blood; 21 bottles (28.8%) were positive. There were three false negative cultures, only one of which contained a blood volume below 0.5 ml. The median time to positivity was 22.4 hours. There was no correlation between blood volume cultured and time to positivity. Neonatal cultures frequently contain less than 0.5 ml of blood. False negative cultures are rare. Neonatal blood culture bottles need to be validated for blood volumes below 0.5 ml.
摘要
开展了一项前瞻性盲法研究，以确定：新生儿用于培养的血量；少量血液是否会导致假阴性结果；以及培养的血量与阳性出现时间之间是否存在时间关系。评估了73个培养瓶。它们所含血液的中位数为0.63毫升。29个培养瓶（39.7%）所含血液少于0.5毫升；21个培养瓶（28.8%）呈阳性。有3例假阴性培养结果，其中只有1个培养瓶中的血量低于0.5毫升。阳性出现的中位时间为22.4小时。培养的血量与阳性出现时间之间无相关性。新生儿培养瓶中经常含有少于0.5毫升的血液。假阴性培养结果很少见。需要对血量低于0.5毫升的新生儿血培养瓶进行验证。
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本文引用的文献
1
Controlled evaluation of the volume of blood cultured in detection of bacteremia and fungemia.血培养体积在检测菌血症和真菌血症中的对照评估
J Clin Microbiol. 1982 Apr;15(4):558-61. doi: 10.1128/jcm.15.4.558-561.1982.
2
Sepsis with coagulase-negative staphylococci in critically ill newborns.危重新生儿凝固酶阴性葡萄球菌败血症
Am J Dis Child. 1983 May;137(5):461-3. doi: 10.1001/archpedi.1983.02140310043012.
3
Clinical laboratory comparison of the 10-ml isolator blood culture system with BACTEC radiometric blood culture media.10毫升隔离器血培养系统与BACTEC放射性血培养培养基的临床实验室比较。
J Clin Microbiol. 1984 Oct;20(4):618-23. doi: 10.1128/jcm.20.4.618-623.1984.
4
The effect of dilution during culture on detection of low concentrations of bacteria in blood.培养过程中的稀释对血液中低浓度细菌检测的影响。
Pediatr Infect Dis. 1984 Jul-Aug;3(4):317-8. doi: 10.1097/00006454-198407000-00008.
5
Volume of blood submitted for culture from neonates.提交用于新生儿血培养的血量。
J Clin Microbiol. 1986 Sep;24(3):353-6. doi: 10.1128/jcm.24.3.353-356.1986.
6
Bacteremia detected by lysis direct plating in a neonatal intensive care unit.在新生儿重症监护病房通过裂解直接平板培养法检测到的菌血症。
J Clin Microbiol. 1990 Jan;28(1):1-4. doi: 10.1128/jcm.28.1.1-4.1990.
7
Coagulase-negative staphylococcal infections in neonates.新生儿凝固酶阴性葡萄球菌感染
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8
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9
Evaluation of blood culture procedures in a pediatric hospital.一家儿科医院血培养程序的评估
J Clin Microbiol. 1979 Jan;9(1):88-92. doi: 10.1128/jcm.9.1.88-92.1979.
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