Backe J, Roos T, Mulfinger L, Martius J
Department of Obstetrics and Gynecology, University of Würzburg, Germany.
Arch Gynecol Obstet. 1997;259(2):69-77. doi: 10.1007/BF02505312.
The histopathologic features of 855 cervical biopsies were correlated with the presence of human papillomavirus DNA using in situ hybridization (ISH) with biotin labeled type specific probes for Human Papilloma Virus (HPV) types 6, 11, 16, 18, 31, 33 and 51. HPV-DNA was found in 18% (13/72) of cervical intraepithelial neoplasia I (CIN I), 30% (35/115) of CIN II, 28% (57(206) of CIN III, in 84% (21/25) of flat condyloma and in 13% (15/112) of normal cervical tissue. HPV DNA was detectable in 11% (5/46) of cervical adenocarcinoma and in 21% (59/279) of squamous cell carcinoma (SCC) of the cervix. High risk HPV types were identified more often than low risk HPV types in CIN I, CIN II, CIN III and SCC. HPV type 16/18 predominates over HPV types 31/33/51 in CIN I, flat condyloma and in SCC. The prevalence of HPV was strongly associated with the grade of differentiation of SCC. It was identified in 59% (23/39) of well differentiated SCC, in 18% (25/142) of moderately differentiated and in 11% (11/98) of poorly differentiated SCC.
采用原位杂交（ISH）技术，使用生物素标记的针对人乳头瘤病毒（HPV）6、11、16、18、31、33和51型的型特异性探针，将855例宫颈活检的组织病理学特征与人类乳头瘤病毒DNA的存在情况进行关联分析。在宫颈上皮内瘤变I（CIN I）中，18%（13/72）检测到HPV-DNA；CIN II中为30%（35/115）；CIN III中为28%（57/206）；扁平湿疣中为84%（21/25）；正常宫颈组织中为13%（15/112）。在宫颈腺癌中，11%（5/46）可检测到HPV DNA；宫颈鳞状细胞癌（SCC）中为21%（59/279）。在CIN I、CIN II、CIN III和SCC中，高危型HPV的检出率高于低危型HPV。在CIN I、扁平湿疣和SCC中，HPV 16/18型比HPV 31/33/51型更为常见。HPV的患病率与SCC的分化程度密切相关。在高分化SCC中，HPV检出率为59%（23/39）；中分化为18%（25/142）；低分化为11%（11/98）。
