Tencer J, Thysell H, Andersson K, Grubb A
Department of Nephrology, Lund University Hospital, Sweden.
Scand J Urol Nephrol. 1997 Feb;31(1):67-71. doi: 10.3109/00365599709070305.
The stability of albumin, protein HC, immunoglobulin G, kappa- and lambda-chain immunoreactivity, orosomucoid and alpha 1-antitrypsin in urine stored at -20 degrees C for up to 24 months was investigated. Significant decreases of the median concentration values for protein HC, IgG and alpha 1-antitrypsin were observed for native urine. Addition to urine of a preservative solution containing benzamidinium chloride, EDTA, tris(hydroxymethyl)-aminomethane and azide prevented the decreases of the concentration values for protein HC and IgG but not for alpha 1-antitrypsin. The median concentration values for albumin, orosomucoid and kappa- and lambda-chain immunoreactivity did not change significantly upon storage of native urine, nor for urine with the preservative solution.
研究了白蛋白、蛋白HC、免疫球蛋白G、κ和λ链免疫反应性、类粘蛋白和α1抗胰蛋白酶在-20℃储存长达24个月的尿液中的稳定性。对于天然尿液，观察到蛋白HC、IgG和α1抗胰蛋白酶的中位浓度值显著下降。向尿液中添加含有苯甲脒氯化物、EDTA、三（羟甲基）氨基甲烷和叠氮化物的保存溶液可防止蛋白HC和IgG浓度值下降，但不能防止α1抗胰蛋白酶浓度值下降。天然尿液储存时，白蛋白、类粘蛋白以及κ和λ链免疫反应性的中位浓度值没有显著变化，添加保存溶液的尿液也是如此。
