Ferguson E V, Ward A C, Sanglier J J, Goodfellow M
Department of Microbiology, Medical School, Newcastle-upon-Tyne, UK.
Zentralbl Bakteriol. 1997 Jan;285(2):169-81. doi: 10.1016/s0934-8840(97)80025-4.
Curie-point pyrolysis mass spectrometry was used to evaluate the taxonomic integrity of three subclusters, provisionally labelled S. albidoflavus, S. anulatus and S. halstedii, which formed a species-group in an extensive numerical phenetic survey of the genus Streptomyces. Excellent agreement was found between the results of the triplicate analyses of each strain while the duplicated strains clustered adjacent to one another. Sequential principal component-canonical variates analysis of the experimental data collected on the 32 representative organisms showed that the S. albidoflavus strains formed a distinct group. This result taken together with earlier chemical, molecular and numerical taxonomic data indicates that the S. albidoflavus subcluster corresponds to a distinct species. In contrast, the subclusters equated with S. anulatus and S. halstedii were found to be heterogeneous and hence in need of further study. However, it is evident from the present investigation that Curie-point pyrolysis mass spectrometry provides a rapid and reproducible way of evaluating the taxonomic integrity of Streptomyces species-groups.
居里点热解质谱法用于评估三个亚群的分类完整性，这三个亚群暂定为白色黄链霉菌、环链霉菌和哈氏链霉菌，它们在对链霉菌属进行的广泛数值分类调查中形成了一个物种组。对每个菌株进行的三次重复分析结果之间发现了极好的一致性，而重复菌株彼此相邻聚类。对32种代表性生物体收集的实验数据进行的顺序主成分-典型变量分析表明，白色黄链霉菌菌株形成了一个独特的组。这一结果与早期的化学、分子和数值分类数据一起表明，白色黄链霉菌亚群对应于一个独特的物种。相比之下，等同于环链霉菌和哈氏链霉菌的亚群被发现是异质的，因此需要进一步研究。然而，从目前的研究中可以明显看出，居里点热解质谱法提供了一种快速且可重复的方法来评估链霉菌物种组的分类完整性。
