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利用传统检测方法和热解质谱法对血链球菌、缓症链球菌及类似微生物进行数值分类学研究。

A numerical taxonomic study of Streptococcus sanguis, S. mitis and similar organisms using conventional tests and pyrolysis mass spectrometry.

作者信息

Magee J T, Hindmarch J M, Douglas C W

机构信息

Department of Bacteriology, Royal Hallamshire Hospital, Sheffield, UK.

出版信息

Zentralbl Bakteriol. 1997 Jan;285(2):195-203. doi: 10.1016/s0934-8840(97)80027-8.

DOI:10.1016/s0934-8840(97)80027-8
PMID:9060152
Abstract

Numerical taxonomic methods were applied to the results of pyrolysis mass spectrometry (Py-MS) and conventional test reaction patterns (CTRPs) obtained with 46 strains of oral streptococci identified as representative of six species. Of these: Streptococcus gordonii strains formed a single homogeneous well-differentiated cluster both in the Py-MS and CTRP analyses; S. oralis strains were more diverse, forming 2 CTRP and 3 Py-MS clusters; S. crista, S. parasanguis and S. sanguis formed clear, but diverse and poorly differentiated clusters on the basis of CTRPs, and were poorly differentiated in the Py-MS analysis; and S. mitis strains were clearly diverse in both analyses. Peritrichously fimbriate ('tufted') S. mitis strains formed a tight cluster, well differentiated from the type strain in both Py-MS and CTRP analyses, and may represent a previously unrecognised centre of variation.

摘要

数值分类方法应用于46株口腔链球菌的热解质谱(Py-MS)结果和常规测试反应模式(CTRP),这些菌株被鉴定为六个物种的代表。其中:戈登链球菌菌株在Py-MS和CTRP分析中均形成了一个单一的、均匀的、分化良好的簇;口腔链球菌菌株更为多样,形成了2个CTRP簇和3个Py-MS簇;嵴链球菌、副血链球菌和血链球菌基于CTRP形成了清晰但多样且分化不佳的簇,在Py-MS分析中分化也不佳;缓症链球菌菌株在两种分析中均明显多样。周身有菌毛(“簇状”)的缓症链球菌菌株形成了一个紧密的簇,在Py-MS和CTRP分析中均与模式菌株有很好的区分,可能代表了一个以前未被认识的变异中心。

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