Rudney J D, Larson C J
Department of Oral Science, School of Dentistry, University of Minnesota, Minneapolis 55455.
J Clin Microbiol. 1994 Feb;32(2):437-43. doi: 10.1128/jcm.32.2.437-443.1994.
This study evaluated restriction fragment length polymorphisms of rRNA genes (ribotyping) for genotypic identification of 53 oral isolates classified as "Streptococcus sanguis" by colony morphology. Isolates were from 8-h buccal plaque on lower first permanent molars of 20 subjects. DNA was digested with AatII and hybridized with digoxygenin-labeled cDNA of Escherichia coli 16S and 23S rRNA. Strains were ribotyped again with AlwNI or PvuII on the basis of the presence or absence of a 2,290-bp AatII band. Band patterns were compared with reference ribotypes for Streptococcus gordonii, Streptococcus sanguis, Streptococcus crista, Streptococcus oralis, Streptococcus mitis, and Streptococcus parasanguis strains. Forty-eight isolates could be assigned to a species (22 S. sanguis, 14 S. oralis, 12 S. gordonii). Multiple species were seen in 14 subjects; multiple strains of the same species occurred in 11 subjects. Our findings suggest that ribotyping can be used for genotypic identification of S. sanguis, S. oralis, and S. gordonii isolates.
本研究评估了rRNA基因的限制性片段长度多态性（核糖体分型），用于对53株通过菌落形态分类为“血链球菌”的口腔分离株进行基因分型鉴定。分离株来自20名受试者下颌第一恒磨牙上8小时的颊菌斑。用AatII消化DNA，并与地高辛标记的大肠杆菌16S和23S rRNA的cDNA杂交。根据是否存在2290 bp的AatII条带，用AlwNI或PvuII再次对菌株进行核糖体分型。将条带模式与戈登链球菌、血链球菌、嵴链球菌、口腔链球菌、缓症链球菌和副血链球菌菌株的参考核糖体分型进行比较。48株分离株可归为一个种（22株血链球菌、14株口腔链球菌、12株戈登链球菌）。14名受试者中观察到多个种；11名受试者中出现同一物种的多个菌株。我们的研究结果表明，核糖体分型可用于血链球菌、口腔链球菌和戈登链球菌分离株的基因分型鉴定。
