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利用热解质谱法对鱼类病原菌鳗弧菌(弧菌属)进行分型

Typing of the fish pathogen Listonella (Vibrio) anguillara by pyrolysis mass spectrometry.

作者信息

Manfio G P, Goodfellow M, Austin B, Austin D A, Pedersen K, Larsen J L, Verdonck L, Swings J

机构信息

Department of Microbiology, Medical School, University of Newcastle-upon-Tyne, UK.

出版信息

Zentralbl Bakteriol. 1997 Jan;285(2):245-51. doi: 10.1016/s0934-8840(97)80031-x.

DOI:10.1016/s0934-8840(97)80031-x
PMID:9060156
Abstract

Twenty-eight representatives of Listonella (Vibrio) anguillara serovars O1, O2 and O3 were compared by Curie-point pyrolysis mass spectrometry (PyMS). The representatives of serovars O1 and O3 formed discrete, homogeneous groups in ordination plots of the PyMS data. Strains from serovar O2 were recovered in two groups, one of which encompassed six strains including the type strain of the species and the reference strain for serovar O2, and the other included two strains which showed cross-reactions between serovars O2 and O5. The almost complete agreement found between the PyMS and the serological data suggests that pyrolysis mass spectrometry will prove to be an effective method for interstrain comparison within the species Listonella anguillara.

摘要

采用居里点热解质谱法(PyMS)对28株鳗弧菌(利斯顿氏菌属)O1、O2和O3血清型的代表菌株进行了比较。在PyMS数据的排序图中,O1和O3血清型的代表菌株形成了离散的、同质的组。O2血清型的菌株分为两组,其中一组包含6株菌株,包括该物种的模式菌株和O2血清型的参考菌株,另一组包括2株在O2和O5血清型之间出现交叉反应的菌株。PyMS与血清学数据之间几乎完全一致,这表明热解质谱法将被证明是鳗弧菌种内菌株间比较的有效方法。

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