Sisson P R, Freeman R, Gould F K, Lightfoot N F
Department of Microbiology, Regional Public Health Laboratory, Newcastle upon Tyne.
J Hosp Infect. 1991 Oct;19(2):137-40. doi: 10.1016/0195-6701(91)90106-i.
Pyrolysis mass spectrometry (PyMS) was used for rapid interstrain comparison of Pseudomonas aeruginosa isolates from a small outbreak of sternal wound infections in a cardiothoracic surgical ward. The PyMS results were compared with those obtained by conventional O-serotyping. All the isolates were phage non-typable. Evidence obtained from PyMS of identity/non-identity between isolates correlated completely with that obtained by O-typing and correctly identified those isolates comprising the epidemic strain, in one instance in advance of O-serotyping. The speed and versatility of PyMS make it an attractive technique for the initial screening of isolates from nosocomial outbreaks of infection with P. aeruginosa and other organisms.
热解质谱法（PyMS）用于对心胸外科病房一次小型胸骨伤口感染暴发中分离出的铜绿假单胞菌菌株进行快速菌株间比较。将PyMS结果与传统O血清分型法获得的结果进行比较。所有分离株均无法进行噬菌体分型。从PyMS获得的菌株间同一性/非同一性证据与O分型法获得的证据完全相关，并正确识别出构成流行菌株的那些分离株，在一个实例中比O血清分型法提前识别出来。PyMS的速度和多功能性使其成为一种有吸引力的技术，可用于对铜绿假单胞菌和其他微生物医院感染暴发中分离出的菌株进行初步筛选。
