小鼠Spam1基因定位于近端6号染色体，是Rb(6.16)24Lub和Rb(6.15)1Ald杂合子精子功能障碍的一个候选基因。

The mouse Spam1 maps to proximal chromosome 6 and is a candidate for the sperm dysfunction in Rb(6.16)24Lub and Rb(6.15)1Ald heterozygotes.

作者信息

Deng X, Moran J, Copeland N G, Gilbert D J, Jenkins N A, Primakoff P, Martin-DeLeon P A

机构信息

Department of Biology, University of Delaware, Newark 19716-2590, USA.

出版信息

Mamm Genome. 1997 Feb;8(2):94-7. doi: 10.1007/s003359900365.

DOI:10.1007/s003359900365

PMID:9060406

Abstract

We have determined the chromosomal localization of the murine gene encoding the 68-kDa sperm adhesion molecule 1, Spam1 or Ph-20. Using two independent approaches, fluorescence in situ hybridization (FISH) and interspecific backcross analysis we show the Spam1 maps to proximal mouse Chromosome (Chr) 6. This map position is within the conserved linkage group corresponding to human Chr 7q, where the human homolog, SPAM 1, has been shown to map previously. Genetic mapping shows the gene to be very closely linked to Met, one of the most proximal loci on MMU 6. It thus places the gene near the centromere and the junction of the Rb(6.16)24Lub and Rb(6.15)1Ald translocations. The essential role of the Spam1 sperm antigen in mouse sperm-egg interactions and its gene location provide strong support for its candidacy as the gene involved in the dysfunction of mouse sperm bearing the Rb(6.16)24Lub or Rb(6.15)1Ald translocation.

摘要

我们已经确定了编码68 kDa精子黏附分子1（Spam1或Ph-20）的小鼠基因的染色体定位。通过两种独立的方法，即荧光原位杂交（FISH）和种间回交分析，我们发现Spam1定位于小鼠近端6号染色体（Chr）。该图谱位置位于与人类7号染色体长臂（Chr 7q）相对应的保守连锁群内，之前已证明人类同源基因SPAM 1定位于此。遗传图谱显示该基因与Met紧密连锁，Met是小鼠6号染色体上最靠近近端的位点之一。因此，该基因位于着丝粒附近以及Rb(6.16)24Lub和Rb(6.15)1Ald易位的交界处。Spam1精子抗原在小鼠精卵相互作用中的重要作用及其基因定位，为其作为与携带Rb(6.16)24Lub或Rb(6.15)1Ald易位的小鼠精子功能障碍相关基因的候选基因提供了有力支持。