Bhattacharyya A, Blackburn E H
Department of Microbiology and Immunology, University of California, San Francisco, CA 94143-0414, USA.
Nucleic Acids Res. 1997 Apr 1;25(7):1426-31. doi: 10.1093/nar/25.7.1426.
We report the identification and cloning of the telomeres of the filamentous fungus,Aspergillus nidulans. We have identified three classes of cloned chromosomal ends based on the telomere-associated sequences (TASs) and demonstrated that the telomeric repeat sequence is TTAGGG, identical to that found in vertebrates, including humans, and some lower eukaryotes. One category of telomere clones was found to contain internal, variant TAAGGG repeats. The A.nidulans telomeric tract length is strikingly short (4-22 repeats). We demonstrate that telomere length is remarkably stable in different cell types and at altered growth temperatures, suggesting a highly regulated mechanism for length control.
我们报告了丝状真菌构巢曲霉端粒的鉴定与克隆。我们基于端粒相关序列（TASs）鉴定出了三类克隆的染色体末端，并证明端粒重复序列为TTAGGG，与在包括人类在内的脊椎动物以及一些低等真核生物中发现的序列相同。一类端粒克隆被发现含有内部可变的TAAGGG重复序列。构巢曲霉的端粒序列长度非常短（4 - 22个重复序列）。我们证明端粒长度在不同细胞类型以及生长温度改变时都非常稳定，这表明存在一种高度调控的长度控制机制。
