Kusumoto K-I, Suzuki S, Kashiwagi Y
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki, 305-8642, Japan.
Appl Microbiol Biotechnol. 2003 May;61(3):247-51. doi: 10.1007/s00253-002-1193-3. Epub 2003 Jan 14.
Four telomeres in the chromosomes of Aspergillus oryzae NFRI1599 were cloned and sequenced. The telomeric repeat sequence of A. oryzae consisted of dodeca-nucleotides: TTAGGGTCAACA. The length of the telomeric repeat tract was 114-136 bp, which corresponds to 9-11 repeats of the dodeca-nucleotide sequence. Compared to a chromosome internal control (18S rDNA), the telomeric sequences were found to be sensitive to BAL31 exonuclease digestion, thus proving that the identified telomeric repeat sequences were located at the most terminal tract of the chromosomes. The length of the telomeric repeat tract of A. oryzae is similar to that of Aspergillus nidulans, whose repeat unit is TTAGGG, indicating that the regulatory mechanism of telomere length might be conserved among Aspergillus species.
对米曲霉NFRI1599染色体中的四个端粒进行了克隆和测序。米曲霉的端粒重复序列由十二核苷酸组成：TTAGGGTCAACA。端粒重复序列的长度为114 - 136 bp，相当于十二核苷酸序列的9 - 11个重复。与染色体内部对照（18S rDNA）相比，发现端粒序列对BAL31核酸外切酶消化敏感，从而证明所鉴定的端粒重复序列位于染色体的最末端区域。米曲霉端粒重复序列的长度与构巢曲霉相似，后者的重复单元是TTAGGG，这表明端粒长度的调控机制可能在曲霉属物种中是保守的。
