从欧洲赤松中克隆与脊椎动物3-羟基-3-甲基戊二酰辅酶A合酶具有高度序列相似性的臭氧诱导蛋白。
Molecular cloning of ozone-inducible protein from Pinus sylvestris L. with high sequence similarity to vertebrate 3-hydroxy-3-methylglutaryl-CoA-synthase.
作者信息
Wegener A, Gimbel W, Werner T, Hani J, Ernst D, Sandermann H
机构信息
GSF-National Research Center for Environment and Health, Institute of Biochemical Plant Pathology, Neuherberg, Germany.
出版信息
Biochim Biophys Acta. 1997 Feb 28;1350(3):247-52. doi: 10.1016/s0005-2760(96)00161-0.
We have isolated a Pinus sylvestris cDNA encoding a globular protein of 474 amino acids with a predicted molecular weight of 52,995 Da. The deduced amino acid sequence showed 41.9% identity and 13.6% similarity to mammalian cytosolic 3-hydroxy-3-methylglutaryl-CoA-synthase (HMGS). Treatment of Scots pine seedlings with ozone resulted in a transient increase of a 1.95 kb transcript, whereas a 1.2 kb mRNA decreased transiently, indicating a possible influence of ozone on isoprenoid biosynthesis.
我们分离出了一种欧洲赤松的cDNA，它编码一种含474个氨基酸的球状蛋白，预测分子量为52,995道尔顿。推导的氨基酸序列与哺乳动物胞质3-羟基-3-甲基戊二酰辅酶A合酶（HMGS）的一致性为41.9%，相似性为13.6%。用臭氧处理欧洲赤松幼苗导致1.95 kb转录本短暂增加，而1.2 kb的mRNA则短暂减少，这表明臭氧可能对类异戊二烯生物合成有影响。
Zotero 插件