Prien D, Blaschke G
Institute of Pharmaceutical Chemistry, University of Münster, Germany.
J Chromatogr B Biomed Sci Appl. 1997 Jan 24;688(2):309-18. doi: 10.1016/s0378-4347(96)00311-8.
A reversed-phase high-performance liquid chromatography method for the determination of dimethindene and its main metabolites N-demethyldimethindene, 6-hydroxydimethindene and 6-hydroxy-N-demethyldimethindene in human urine was developed. The assay was also applied to the quantification of dimethindene-N-oxide in rat urine. Conjugates of the hydroxylated metabolites were determined after enzymatic deconjugation. Moreover the direct determination of dimethindene and its metabolites without prior extraction from urine was performed by capillary electrophoresis. The direct simultaneous determination of the enantiomers of dimethindene and N-demethyldimethindene was achieved on a Chiralcel OD column. Urinary data after oral administration of dimethindene are presented. The assays were used to study dimethindene and it metabolites in urine upon oral administration of the drug to rats and human volunteers.
建立了一种反相高效液相色谱法，用于测定人尿中二甲茚定及其主要代谢产物N-去甲基二甲茚定、6-羟基二甲茚定和6-羟基-N-去甲基二甲茚定。该方法也用于定量大鼠尿液中的二甲茚定-N-氧化物。经酶解后测定羟基化代谢产物的缀合物。此外，通过毛细管电泳对二甲茚定及其代谢产物进行了无需预先从尿液中提取的直接测定。在Chiralcel OD柱上实现了二甲茚定和N-去甲基二甲茚定对映体的直接同时测定。给出了口服二甲茚定后的尿液数据。这些分析方法用于研究给大鼠和人类志愿者口服该药物后尿液中的二甲茚定及其代谢产物。
