Novotny M V
Department of Chemistry, Indiana University, Bloomington 47405, USA.
J Chromatogr B Biomed Sci Appl. 1997 Feb 7;689(1):55-70. doi: 10.1016/s0378-4347(96)00398-2.
This article summarizes the recent advances in microcolumn separations of biopolymers. Microcolumn liquid chromatography is primarily emphasized for its role as a micropreparative and fractionation tool, whereas high-performance capillary electrophoresis is demonstrated as a highly efficient technique for final analyses. Following a brief discussion of new trends in instrumentation, the recent applications of capillary techniques to proteins, DNA and glycoconjugates are reviewed.
本文总结了生物聚合物微柱分离技术的最新进展。微柱液相色谱主要强调其作为微量制备和分级分离工具的作用，而高效毛细管电泳则被证明是一种用于最终分析的高效技术。在简要讨论仪器的新趋势之后，本文综述了毛细管技术在蛋白质、DNA和糖缀合物方面的最新应用。
