de Zeeuw R A
Department of Analytical Chemistry and Toxicology, University Centre for Pharmacy, Groningen, Netherlands.
J Chromatogr B Biomed Sci Appl. 1997 Feb 7;689(1):71-9. doi: 10.1016/s0378-4347(96)00332-5.
In this paper the key steps towards drug screening in biological fluids are considered: (i) sample work up-isolation-concentration; (ii) differentiation-detection; (iii) identification. For (i) solid-phase extraction has very good potential; for (ii) thin-layer chromatography, gas chromatography and high-performance liquid chromatography, in combination with their respective detection modes, each have their particular advantages and disadvantages; for (iii) extensive computerized data bases are essential. It is emphasized that each step must be approached and carried out in a systematic way, not only to make sure that all substances of interest can be detected, but also for their correct identification. To this end all analytical procedures and techniques used require extensive validation of their detection and identification properties.
（i）样品预处理——分离——浓缩；（ii）鉴别——检测；（iii）鉴定。对于（i），固相萃取具有很大潜力；对于（ii），薄层色谱法、气相色谱法和高效液相色谱法，结合各自的检测模式，各有其优缺点；对于（iii），广泛的计算机数据库至关重要。需要强调的是，每个步骤都必须以系统的方式进行，这不仅是为了确保所有感兴趣的物质都能被检测到，也是为了对其进行正确鉴定。为此，所使用的所有分析程序和技术都需要对其检测和鉴定特性进行广泛验证。
