细胞培养物中一氧化氮合酶活性检测的组织化学方法。
Histochemical method for detecting nitric oxide synthase activity in cell cultures.
作者信息
Hon W M, Chhatwal V J, Khoo H E, Moochhala S M
机构信息
Department of Biochemistry, National University of Singapore, Singapore.
出版信息
Biotech Histochem. 1997 Jan;72(1):29-32. doi: 10.3109/10520299709082208.
NADPH diaphorase histochemistry has been used extensively for detecting nitric oxide synthase (NOS) activity in various cell types including neuronal cell bodies, vascular endothelium, cells of the immune system and epithelial cells. The use of the diaphorase technique in cell cultures to study the induction of NOS has not been investigated. In this paper we report the use of diaphorase histochemistry as a good marker for the detection of NOS activity in cultured cells. This technique can be used in conjunction with other established techniques to determine the presence and activity of NOS in cultured cells.
还原型辅酶Ⅱ黄递酶组织化学已被广泛用于检测多种细胞类型中的一氧化氮合酶（NOS）活性，这些细胞类型包括神经元细胞体、血管内皮、免疫系统细胞和上皮细胞。尚未研究在细胞培养中使用黄递酶技术来研究NOS的诱导情况。在本文中，我们报告了使用黄递酶组织化学作为检测培养细胞中NOS活性的良好标志物。该技术可与其他既定技术结合使用，以确定培养细胞中NOS的存在和活性。
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