Cicciarello S, Borgia G, Crowell J, Ciampi R, Cerini R, Orlando R, Mainolfi M, Reynaud L, Milano M, Piazza M
Institute of Infectious Diseases, University of Naples Federico II, Italy.
Eur J Epidemiol. 1997 Jan;13(1):49-54. doi: 10.1023/a:1007324300358.
HCV is ubiquitous. In 50% of all cases it causes chronic hepatitis that often evolves into liver cirrhosis and hepatocellular carcinoma. Recently HCV has been classified in 5 genotypes by Okamoto. The purpose of this study is to evaluate the prevalence of 5 genotypes in Campania, a region of southern Italy, where the prevalence of anti-HCV antibodies ranges from 0.87 to 4%, and to evaluate the correlation between the HCV genotypes and the severity of histological damage. One-hundred- and-thirty-five anti-HCV positive patients were enrolled and tested by PCR to identify HCV-RNA. One-hundred-and-twenty-four patients resulted HCV-RNA positive. Genotyping was performed as described by Okamoto et al. with minor modifications of the specific primer to type III proposed by Silini et al. Eight patients were negative for all genotypes. Eight patients were positive for type I(1a), 61 for type II(1b), 39 for type III(2a), 11 for type IV(2b) and 1 for type V(3a). In 4 cases two different genotypes were present in the same sample [II(1b)-IV(2b), III(2a)-II(1b) twice, III (2a)-IV(2b)]. Histological evaluation of liver damage showed: CPH (22 cases), minimal CAH (56), severe CAH (31) and liver cirrhosis (15). There was no statistically significant correlation between the 5 genotypes and the severity of histological damage. Data on the prevalence of genotype II (1b) in Italy are similar to those reported for other European countries. The prevalence of genotypes in southern Italy is similar to that reported in the population of northern Italy.
丙型肝炎病毒（HCV）广泛存在。在所有病例中，50%会引发慢性肝炎，且常常发展为肝硬化和肝细胞癌。最近，冈本将HCV分为5种基因型。本研究的目的是评估意大利南部坎帕尼亚地区5种基因型的流行情况，该地区抗-HCV抗体的流行率在0.87%至4%之间，并评估HCV基因型与组织学损伤严重程度之间的相关性。招募了135名抗-HCV阳性患者，并通过聚合酶链反应（PCR）检测以鉴定HCV-RNA。124名患者HCV-RNA呈阳性。按照冈本等人所述方法进行基因分型，并对西里尼等人提出的III型特异性引物进行了微小修改。8名患者所有基因型均为阴性。8名患者I型（1a）呈阳性，61名患者II型（1b）呈阳性，39名患者III型（2a）呈阳性，11名患者IV型（2b）呈阳性，1名患者V型（3a）呈阳性。在4个病例中，同一样本中存在两种不同的基因型[II（1b）-IV（2b）、III（2a）-II（1b）两次、III（2a）-IV（2b）]。肝脏损伤的组织学评估显示：慢性持续性肝炎（CPH，22例）、轻度慢性活动性肝炎（CAH，56例）、重度CAH（31例）和肝硬化（15例）。5种基因型与组织学损伤严重程度之间无统计学显著相关性。意大利II型（1b）基因型的流行数据与其他欧洲国家报告的数据相似。意大利南部基因型的流行情况与意大利北部人群报告的情况相似。
