Yamada M, Fukuda Y, Imoto M, Koyama Y, Hayakawa T, Takayama T, Nagura H
Second Department of Internal Medicine, Nagoya University School of Medicine, Aichi, Japan.
J Gastroenterol Hepatol. 1994 Nov-Dec;9(6):620-3. doi: 10.1111/j.1440-1746.1994.tb01571.x.
The subjects of this study were 151 patients (69 males and 82 females) who underwent examination and liver biopsy owing to liver dysfunction in an epidemic area with hepatitis C. Second generation hepatitis C virus antibody (HCV Ab) was positive in 116 (76.8%) of 151 cases. HCV-RNA was detected in 120 (79.5%) by polymerase chain reaction (PCR). In 7 (4.6%) cases, HCV Ab could not be found, but HCV-RNA was detected. Three (2.0%) cases were positive for HCV Ab but negative for HCV-RNA. On the basis of variation in nucleotide sequence within a restricted region in the putative core gene of HCV, HCV genotypes were classed into four types of I, II, III and IV by PCR. The genotypes were identified in 120 cases. Ninety-eight (81.7%) cases carried one of the four types. Type II was found in 76 (63.3%) cases and type III in 22 (18.3%). The other 22 (18.3%) carried simultaneously two different genotypes (mixed type): 21 (17.5%) cases with type II + III and one (0.8%) case with type II + IV. In comparison with the incidence of HCV mixed types in cases with hepatitis C in a non-epidemic area, carriers of mixed types were found at a significantly higher rate in the epidemic area. Liver biopsy of 120 cases with identified HCV genotypes in the epidemic area showed 93 cases of chronic active hepatitis, nine of chronic lobular hepatitis, 10 of chronic persistent hepatitis and eight of liver cirrhosis. No significant correlation could be detected between liver histology and HCV genotypes.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究的对象为151例患者（69例男性和82例女性），他们因丙型肝炎流行地区的肝功能障碍接受了检查和肝活检。151例中有116例（76.8%）第二代丙型肝炎病毒抗体（HCV Ab）呈阳性。通过聚合酶链反应（PCR）在120例（79.5%）中检测到HCV-RNA。7例（4.6%）中未发现HCV Ab，但检测到HCV-RNA。3例（2.0%）HCV Ab呈阳性但HCV-RNA呈阴性。根据HCV推定核心基因受限区域内核苷酸序列的变异，通过PCR将HCV基因型分为I、II、III和IV四种类型。在120例中鉴定出基因型。98例（81.7%）携带四种类型之一。76例（63.3%）为II型，22例（18.3%）为III型。另外22例（18.3%）同时携带两种不同基因型（混合型）：21例（17.5%）为II + III型，1例（0.8%）为II + IV型。与非流行地区丙型肝炎病例中HCV混合型的发生率相比，流行地区混合型携带者的比例明显更高。对流行地区120例已鉴定HCV基因型的患者进行肝活检，结果显示93例为慢性活动性肝炎，9例为慢性小叶性肝炎，10例为慢性持续性肝炎，8例为肝硬化。未发现肝组织学与HCV基因型之间存在显著相关性。（摘要截断于250字）
