Brown S
Department of Molecular Cell Biology, University of Copenhagen, Denmark.
Nat Biotechnol. 1997 Mar;15(3):269-72. doi: 10.1038/nbt0397-269.
Attachment of proteins to metal surfaces has the potential to improve our understanding of protein adhesion and has applications in sensor technology. Repeating polypeptides able to bind to metallic gold or chromium were selected from a population of approximately 5 million different polypeptides. Each polypeptide contained several direct repeats of identical peptide units 14 or 28 amino acids long. The metal-recognizing polypeptides were found to retain their binding properties when freed from the protein used to select them. One gold-binding polypeptide's avidity for gold was found to be dependent on the number of repeats and the presence of salt.
蛋白质与金属表面的附着有可能增进我们对蛋白质黏附的理解，并在传感器技术中有应用。从大约500万种不同的多肽群体中筛选出了能够与金属金或铬结合的重复多肽。每个多肽包含几个长度为14或28个氨基酸的相同肽单元的直接重复序列。发现这些金属识别多肽在从用于筛选它们的蛋白质中释放出来后仍保留其结合特性。发现一种与金结合的多肽对金的亲和力取决于重复序列的数量和盐的存在。
