Yang K, Edelmann W, Fan K, Lau K, Kolli V R, Fodde R, Khan P M, Kucherlapati R, Lipkin M
Strang Cancer Prevention Center, New York Hospital-Cornell Medical Center, New York 10021, USA.
J Exp Zool. 1997 Feb 15;277(3):245-54.
In an effort to generate a good mouse model for human colorectal cancer, we generated mice which carry a mutation in the adenomatous polyposis coli (Apc) gene. Mice which are heterozygous for the mutation, designated Apc1638, develop colonic polyps and tumors of the small intestine. Neoplasms were found in 96% of animals studied, and they included adenomas, adenocarcinomas, and polypoid hyperplasias. The mice developed an average of 3.3 tumors, with the highest number in duodenum, followed by jejunum, stomach, ileum, and colon. Focal areas of dysplasias were observed in the colonic mucosa in 50% of mice which were 10 months old or older. These results suggest that mice carrying the Apc1638 mutation can serve as a good model to study the initiation, progression, and inhibition of gastrointestinal tumors.
为了构建一种良好的人类结直肠癌小鼠模型，我们培育了携带腺瘤性息肉病 coli（Apc）基因突变的小鼠。携带该突变杂合子的小鼠，命名为 Apc1638，会发展出结肠息肉和小肠肿瘤。在研究的动物中，96%发现了肿瘤，包括腺瘤、腺癌和息肉样增生。这些小鼠平均长出 3.3 个肿瘤，数量最多的是十二指肠，其次是空肠、胃、回肠和结肠。在 10 个月及以上的小鼠中，50%的结肠黏膜观察到发育异常的局灶区域。这些结果表明，携带 Apc1638 突变的小鼠可作为研究胃肠道肿瘤的发生、发展和抑制的良好模型。
