使用玻碳电极进行还原电化学检测的血浆青蒿素高效液相色谱分析。
A high-performance liquid chromatography analysis of plasma artemisinin using a glassy carbon electrode for reductive electrochemical detection.
作者信息
Chan K L, Yuen K H, Jinadasa S, Peh K K, Toh W T
机构信息
School of Pharmaceutical Science, University of Science Malaysia, Penang, Malaysia.
出版信息
Planta Med. 1997 Feb;63(1):66-9. doi: 10.1055/s-2006-957607.
A high-performance liquid chromatography assay equipped with a glassy carbon electrode for electrochemical detection (HPLC-ECD) was developed at reductive mode for the analysis of artemisinin, the antimalarial drug from Artemisia annua (Asteraceae) in human plasma. This method was selective, sensitive, and produced satisfactory recovery, precision, and accuracy. Analysis of plasma samples from 8 male volunteers given 10 mg kg-1 of artemisinin orally as an aqueous suspension showed a mean peak plasma concentration (Cmax) of 580.89 ng ml-1 +/- 88.64 SD at 2.5 h +/- 0.5 SD after dosing, and the mean area under the plasma concentration-time curve (AUC0-infinity) was 2227.57 ng h ml-1 +/- 677.22 SD. In addition, the elimination rate constant (Ke), elimination half-life (t1/2), and apparent volume of distribution (Vd) were calculated to be 0.2971 h-1 +/- 0.0644 SD, 2.42 h +/- 0.46 SD, and 16.26 l kg-1 +/- 3.44 SD, respectively.
开发了一种配备玻碳电极用于电化学检测的高效液相色谱法（HPLC-ECD），以还原模式分析青蒿素，这是一种从菊科植物黄花蒿中提取的抗疟药物，用于检测人体血浆中的含量。该方法具有选择性、灵敏性，回收率、精密度和准确性均令人满意。对8名男性志愿者口服10 mg/kg青蒿素水悬浮液后的血浆样本进行分析，给药后2.5 h±0.5 SD时，血浆平均峰浓度（Cmax）为580.89 ng/ml±88.64 SD，血浆浓度-时间曲线下平均面积（AUC0-∞）为2227.57 ng·h/ml±677.22 SD。此外，消除速率常数（Ke）、消除半衰期（t1/2）和表观分布容积（Vd）分别计算为0.2971 h-1±0.0644 SD、2.42 h±0.46 SD和16.26 l/kg±3.44 SD。
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