脯氨酸是一种蛋白质增溶溶质。

Proline is a protein solubilizing solute.

作者信息

Samuel D, Kumar T K, Jayaraman G, Yang P W, Yu C

机构信息

Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan.

出版信息

Biochem Mol Biol Int. 1997 Feb;41(2):235-42. doi: 10.1080/15216549700201241.

DOI:10.1080/15216549700201241

PMID:9063563

Abstract

The effect of proline on the prevention of trichloroacetic acid (TCA)-induced protein precipitation is studied. It is found that proline at high concentrations (> 4.0 M) completely prevents TCA-induced precipitation of hen egg white lysozyme. Other osmolytes such as ethylene glycol, glycerol and sucrose fail to prevent the TCA-induced precipitation of lysozyme. Viscosity and 1-anilino-8-naphthalene sulphonic acid binding experiments suggest that proline at high concentration forms an ordered supramolecular assembly. Proline is shown to increase the solubility of protein due to formation of such higher order assemblies. A model of the supra-molecular assembly of proline is proposed and a possible in vivo role of the increased levels of proline under water stress is discussed.

摘要

研究了脯氨酸对预防三氯乙酸（TCA）诱导的蛋白质沉淀的作用。发现高浓度（>4.0 M）的脯氨酸可完全防止TCA诱导的鸡蛋清溶菌酶沉淀。其他渗透剂，如乙二醇、甘油和蔗糖，无法防止TCA诱导的溶菌酶沉淀。粘度和1-苯胺基-8-萘磺酸结合实验表明，高浓度的脯氨酸形成有序的超分子组装体。由于形成了这种高阶组装体，脯氨酸被证明可增加蛋白质的溶解度。提出了脯氨酸超分子组装体的模型，并讨论了水分胁迫下脯氨酸水平升高可能在体内发挥的作用。