单个T细胞受体以高特异性识别结构不同的MHC/肽复合物。
A single T cell receptor recognizes structurally distinct MHC/peptide complexes with high specificity.
作者信息
Tallquist M D, Yun T J, Pease L R
机构信息
Department of Immunology, Mayo Foundation, Rochester, Minnesota 55905, USA.
出版信息
J Exp Med. 1996 Sep 1;184(3):1017-26. doi: 10.1084/jem.184.3.1017.
Abstract
The 2C T cell is a CD8+, alloreactive T cell, which recognizes cells bearing Ld and Kbm3 class I major histocompatability complex molecules. Here, we characterize an allopeptide, designated dEV-8, that is a ligand in the Kbm3 molecule for the 2C TCR but is not a ligand in the Ld molecule. By biochemical and immunological properties, dEV-8 is distinct from P2Ca, the Ld allopeptide that is also recognized by the 2C TCR. Using the deduced amino acid sequence of dEV-8, we isolate a candidate endogenous source of the peptide. The endogenous protein, MLRQ, contains a peptide sequence identical to dEV-8. This degenerate recognition of two distinct peptide/MHC complexes by a single TCR has important implications for understanding allorecognition.
摘要
2C T细胞是一种CD8 +的同种异体反应性T细胞，它识别携带Ld和Kbm3 I类主要组织相容性复合体分子的细胞。在此，我们鉴定了一种名为dEV - 8的别位肽，它是Kbm3分子中2C TCR的配体，但不是Ld分子的配体。从生化和免疫学特性来看，dEV - 8与P2Ca不同，P2Ca是Ld别位肽，也能被2C TCR识别。利用dEV - 8推导的氨基酸序列，我们分离出了该肽的候选内源性来源。内源性蛋白质MLRQ包含与dEV - 8相同的肽序列。单个TCR对两种不同肽/MHC复合物的这种简并识别对于理解同种异体识别具有重要意义。
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本文引用的文献
1
The repertoire of T cells shaped by a single MHC/peptide ligand.由单一主要组织相容性复合体/肽配体塑造的T细胞库。
Cell. 1996 Feb 23;84(4):521-9. doi: 10.1016/s0092-8674(00)81028-4.
2
Positive selection of CD8+ T cells induced by major histocompatibility complex binding peptides in fetal thymic organ culture.主要组织相容性复合体结合肽在胎儿胸腺器官培养中诱导CD8 + T细胞的阳性选择。
J Exp Med. 1993 May 1;177(5):1469-73. doi: 10.1084/jem.177.5.1469.
3
The molecular basis of allorecognition.同种异体识别的分子基础。
Annu Rev Immunol. 1993;11:385-402. doi: 10.1146/annurev.iy.11.040193.002125.
4
Peptides naturally presented by MHC class I molecules.由MHC I类分子天然呈递的肽段。
Annu Rev Immunol. 1993;11:213-44. doi: 10.1146/annurev.iy.11.040193.001241.
5
6
Conformational differences in major histocompatibility complex-peptide complexes can result in alloreactivity.主要组织相容性复合体-肽复合物的构象差异可导致同种异体反应性。
J Exp Med. 1994 Jan 1;179(1):213-9. doi: 10.1084/jem.179.1.213.
7
A ubiquitous protein is the source of naturally occurring peptides that are recognized by a CD8+ T-cell clone.一种普遍存在的蛋白质是天然存在的肽的来源，这些肽可被一个CD8 + T细胞克隆识别。
Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11272-6. doi: 10.1073/pnas.90.23.11272.
8
Resting and activated T cells display different requirements for CD8 molecules.静息和活化的T细胞对CD8分子表现出不同的需求。
J Exp Med. 1994 Jun 1;179(6):2005-15. doi: 10.1084/jem.179.6.2005.
9
10
Evidence for a differential avidity model of T cell selection in the thymus.胸腺中T细胞选择的差异亲和力模型的证据。
Cell. 1994 Feb 25;76(4):651-63. doi: 10.1016/0092-8674(94)90505-3.
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