[流感病毒成分中DNA的检测]
[Detection of DNA in the composition of influenza virus].
作者信息
Kantorovich-Prokudina E N, Semenova N P, Berezina O N, Zhdanov V M
出版信息
Vopr Virusol. 1977 May-Jun(3):279-84.
When influenza virus multiplies in chick embryos, incorporation of 3H-thymidine into virus particles is observed. The radioactivity is localized in influenza virus nucleoprotein. The analysis of 3H-thymidine-containing structures in cesium chloride density gradient permits to qualify them as DNA. Isodensity centrifugation of in vitro 125I-labeled preparation of nucleic acid from influenza virus in cesium sulphate gradient also reveals DNA molecules, in addition to RNA. A high hybridization rate of influenza virus 125I-DNA with virion RNA has been demonstrated.
当流感病毒在鸡胚中增殖时，可观察到3H-胸腺嘧啶核苷掺入病毒颗粒。放射性定位于流感病毒核蛋白中。通过氯化铯密度梯度分析含3H-胸腺嘧啶核苷的结构可将其鉴定为DNA。在硫酸铯梯度中对流感病毒核酸的体外125I标记制剂进行等密度离心，除RNA外还揭示了DNA分子。已证明流感病毒125I-DNA与病毒粒子RNA具有高杂交率。
Zotero 插件