Krutilina D V, Morogova V M, Kovliasova E G, Ganiev R M
Vopr Virusol. 1977 May-Jun(3):321-3.
Experimental lyophilized tissue culture rabies vaccine inactivated with gamma-rays was prepared from the Vnukovo-32 strain and showed a sufficiently high immunogenic activity. Complete inactivation of the virus and the immunogenic potency of the vaccine depended upon the dose of irradiation and temperature during irradiation. The virus lost infectivity at 18 degrees C when irradiated with 2.6 X 10(6) r and at --70 degrees C with 4.55 X 10(6) r. However, the immunogenic potency of the vaccine inactivated at minus temperature without thawing was significantly higher.
用伽马射线灭活的实验性冻干组织培养狂犬病疫苗是由伏努科沃-32株制备的，具有足够高的免疫原活性。病毒的完全灭活和疫苗的免疫原效力取决于照射剂量和照射期间的温度。当用2.6×10⁶伦琴照射时，病毒在18℃失去感染性；当在-70℃用4.55×10⁶伦琴照射时，病毒也失去感染性。然而，在低温下不解冻灭活的疫苗免疫原效力明显更高。
