Larghi O P, Nebel A E
J Clin Microbiol. 1980 Feb;11(2):120-2. doi: 10.1128/jcm.11.2.120-122.1980.
The inactivation dynamics of rabies virus (PV strain) by binary ethylenimine, and the immunogenic properites and the stability of the vaccines prepared using this agent, were studied. Binary ethylenimine at a final concentration of 0.01 M was prepared wtih 2-bromoethylamine hydrobromide in alkaline solutions, either separately from or in suspensions of rabies virus propagated in BHK cells. The infectivity of virus suspensions containing more than 108 plaque-forming units per 0.1 ml was inactivated in 2 h when the inactivating agent was prepared before its addition to the suspensions, and in3 h when prepared directly in the suspensions. Liquid vaccines prepared in this manner and stored at different temperatures maintained potency for 1 month at 37 degrees C and for 6 months at 4 degrees C and 22 to 25 degrees C. Lyophilized vaccine maintained its potency for 6 months at the three temperatures. The inactivated vaccine mixed with aluminum or oil adjuvant at high dilutions protected guinea pigs against challenge. This safer procedure for rabies virus inactivation offers promise for the production of effective vaccines for the immunization of dogs and cattle.
研究了双乙基亚胺对狂犬病病毒（PV株）的灭活动力学，以及用该试剂制备的疫苗的免疫原性和稳定性。在碱性溶液中，用氢溴酸2-溴乙胺制备终浓度为0.01M的双乙基亚胺，其制备方式可以是与在BHK细胞中繁殖的狂犬病病毒悬液分开制备，也可以是在其中制备。当灭活剂在加入病毒悬液之前制备时，每0.1ml含超过108个空斑形成单位的病毒悬液的感染性在2小时内被灭活；当直接在悬液中制备时，感染性在3小时内被灭活。以这种方式制备并在不同温度下储存的液体疫苗，在37℃下效力可维持1个月，在4℃和22至25℃下可维持6个月。冻干疫苗在这三个温度下效力可维持6个月。与铝或油佐剂在高稀释度下混合的灭活疫苗可保护豚鼠免受攻击。这种更安全的狂犬病病毒灭活方法为生产用于犬和牛免疫的有效疫苗带来了希望。
