Caccuri R L, Iacono R F, Weissenbacher M C, Avila M M, Berría M I
Department of Microbiology, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina.
J Neural Transm (Vienna). 2003 Aug;110(8):847-57. doi: 10.1007/s00702-003-0010-1.
Immunoperoxidase labeling was performed in histological sections from rat brain harvested during acute (10-30 days), clinically inapparent (90-270 days) and late (450-540 days) stages of Junin virus-induced neurological disease. In frontoparietal cortex, count of viral antigen (+) neurons peaked during the acute period (27.7+/-6.8), dropped within the intermediate (4.8+/-4.0 to 1.4+/-1.1) and increased (7.6+/-4.3) at the onset of the late neurological syndrome. In infected vs. control rats, the number of GFAP (+) astrocytes maximized during the acute stage (19+/-4 vs. 11+/-5), and from the end of the intermediate (27+/-5 vs. 21+/-5) up to the late (37+/-7 vs. 26+/-6) periods. In turn, surface density of GFAP (+) material in infected samples peaked at 0.196+/-0.066, while it failed to exceed 0.090+/-0.043 in controls. Both astrocyte hypertrophy relapsing into chronicity, as depicted by surface density, and astrocyte hyperplasia preceding the onset of the late neurological syndrome, support their pathogenic contribution to disease expression.
对感染胡宁病毒所致神经疾病的大鼠脑部组织切片进行免疫过氧化物酶标记，这些组织切片取自急性（10 - 30天）、临床无症状期（90 - 270天）和晚期（450 - 540天）阶段。在额顶叶皮质，病毒抗原（+）神经元数量在急性期达到峰值（27.7±6.8），在中间期下降（4.8±4.0至1.4±1.1），并在晚期神经综合征发作时增加（7.6±4.3）。在感染组与对照组大鼠中，GFAP（+）星形胶质细胞数量在急性期达到最大值（19±4对11±5），从中间期末期（27±5对21±5）直至晚期（37±7对26±6）均保持较高水平。相应地，感染样本中GFAP（+）物质的表面密度峰值为0.196±0.066，而对照组未超过0.090±0.043。表面密度所显示的星形胶质细胞肥大复归慢性状态以及晚期神经综合征发作前的星形胶质细胞增生，均支持它们在疾病表现中的致病作用。
