出血热病毒感染后反应性星形胶质细胞增生：胡宁病毒感染的人星形胶质细胞的基因芯片分析
Reactive astrogliosis in response to hemorrhagic fever virus: microarray profile of Junin virus-infected human astrocytes.
作者信息
Kolokoltsova Olga A, Yun Nadezhda E, Paessler Slobodan
机构信息
Department of Pathology, Galveston National Laboratory, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX, USA.
出版信息
Virol J. 2014 Jul 11;11:126. doi: 10.1186/1743-422X-11-126.
BACKGROUND
Arenavirus Junin is the causative agent of Argentine hemorrhagic fever. Limited information is available concerning the pathogenesis of this human disease, especially the pathogenesis of acute and late neurological symptoms.
METHODS
In our study we present for the first time cDNA microarray profile of human astrocytes infected with the virulent strain of Junin virus. Transcriptional profiling was confirmed by quantitative real-time RT-PCR and cytokine/chemokine/growth factor assay.
RESULTS
We demonstrated the impact of virus infection on immune/inflammatory response/interferon signaling and apoptosis. Pro-apoptotic response and amplification with time of pro-inflammatory cascade of human astrocytes suggested neurodegenerative dysfunctional reactive astrogliosis in response to Junin virus infection.
CONCLUSION
Our results suggest potential pathogenic role of astroglial cells in the development of neurological symptoms and late neurological syndrome during Argentine hemorrhagic fever.
背景
胡宁病毒是阿根廷出血热的病原体。关于这种人类疾病的发病机制，尤其是急性和晚期神经症状的发病机制，可用信息有限。
方法
在我们的研究中，我们首次展示了感染胡宁病毒强毒株的人星形胶质细胞的cDNA微阵列图谱。通过定量实时逆转录聚合酶链反应和细胞因子/趋化因子/生长因子测定对转录谱进行了确认。
结果
我们证明了病毒感染对免疫/炎症反应/干扰素信号传导和细胞凋亡的影响。人星形胶质细胞促凋亡反应以及促炎级联随时间的放大表明，对胡宁病毒感染的反应存在神经退行性功能失调性反应性星形胶质细胞增生。
结论
我们的结果表明，星形胶质细胞在阿根廷出血热期间神经症状和晚期神经综合征的发展中可能具有致病作用。
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