Mizumoto Y, Mizuta H, Nakamura E, Takagi K
Department of Othopaedic Surgery, Kumamoto University School of Medicine, Japan.
Acta Orthop Scand. 1996 Dec;67(6):562-5. doi: 10.3109/17453679608997756.
We compared the effect of various distraction frequencies on the gastrocnemius muscle by evaluating the histological findings, intramuscular enzyme contents, and DNA contents. In 15 rabbits, both tibiae were distracted 1 mm per day. The distraction frequency was 2 steps (0.5 mm/12 hour) by hand on the right side and 120 steps (0.0083 mm/12 minutes) by an auto-distractor on the left. The rabbits were divided into 3 subgroups based on length gain: 10%, 20%, and 30%. Histologically, there were no signs of fibrosis or edema and no differences in the number of necrotic cells, and intramuscular enzyme contents between the 2- and 120-step groups. The DNA content, however, was higher in the 120-step group at 30% lengthening in the middle of the muscle belly, and at 20% and 30% lengthening in the musculotendinous junction. Our findings suggest that an increase in the distraction frequency may promote DNA synthesis in the muscle, thus providing better muscle accommodation during bone lengthening.
我们通过评估组织学结果、肌肉内酶含量和DNA含量，比较了不同牵引频率对腓肠肌的影响。在15只兔子中，双侧胫骨均以每天1毫米的速度进行牵引。右侧通过手动牵引，牵引频率为2步（0.5毫米/12小时），左侧通过自动牵引器牵引，牵引频率为120步（0.0083毫米/12分钟）。根据长度增加情况，将兔子分为3个亚组：10%、20%和30%。组织学上，2步组和120步组之间没有纤维化或水肿迹象，坏死细胞数量以及肌肉内酶含量也没有差异。然而，在肌腹中部延长30%时，以及在肌腱结合部延长20%和30%时，120步组的DNA含量更高。我们的研究结果表明，牵引频率的增加可能会促进肌肉中的DNA合成，从而在骨延长过程中提供更好的肌肉适应性。
