绝经后女性血清胰岛素样生长因子结合蛋白谱：其与骨密度的相关性
Serum insulin-like growth factor binding protein profiles in postmenopausal women: their correlation with bone mineral density.
作者信息
Kim J G, Lee J Y
机构信息
Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Korea.
出版信息
Am J Obstet Gynecol. 1996 May;174(5):1511-7. doi: 10.1016/s0002-9378(96)70598-7.
OBJECTIVE
The objective of this study was to evaluate the relationship between serum insulin-like growth factor binding protein profiles and bone mineral density measurements and biochemical markers of bone turnover in postmenopausal women.
STUDY DESIGN
Sera from 37 normal healthy postmenopausal women and 14 postmenopausal women with osteoporosis were analyzed for insulin-like growth factor binding proteins and osteocalcin by Western ligand blotting and radioimmunoassay, respectively. Bone mineral density measurements were performed by dual-energy x-ray absorptiometry. Urinary calcium and creatinine excretion were also determined. The data were analyzed statistically by analysis of variance and the least-squares method.
RESULTS
The serum insulin-like growth factor binding protein-2 ratio (insulin-like growth factor binding protein-2 intensity/total insulin-like growth factor binding protein intensity) in postmenopausal women with osteoporosis was significantly higher (p < 0.02) than that in normal healthy postmenopausal women, but the serum insulin-like growth factor binding protein-3 ratio in women with osteoporosis was significantly lower (p < 0.01). The serum insulin-like growth factor binding protein-2 ratio correlated negatively with the bone mineral density of the lumbar spine (p < 0.0001), femoral neck (p < 0.05), trochanter (p < 0.01), and Ward's triangle (p < 0.05), but there was a significant positive correlation between the serum insulin-like growth factor binding protein-3 ratio and the bone mineral density of the lumbar spine (p < 0.001) and trochanter (p < 0.05). No correlations between serum insulin-like growth factor binding protein levels or serum ratios and biochemical markers of bone turnover were noted.
CONCLUSION
The measurement of serum insulin-like growth factor binding protein profiles might be useful in identifying postmenopausal women at risk for osteoporosis.
目的
本研究的目的是评估绝经后女性血清胰岛素样生长因子结合蛋白谱与骨密度测量值以及骨转换生化标志物之间的关系。
研究设计
分别采用Western配体印迹法和放射免疫分析法，对37名正常健康绝经后女性和14名绝经后骨质疏松女性的血清进行胰岛素样生长因子结合蛋白和骨钙素分析。采用双能X线吸收法进行骨密度测量。同时测定尿钙和肌酐排泄量。数据采用方差分析和最小二乘法进行统计学分析。
结果
绝经后骨质疏松女性的血清胰岛素样生长因子结合蛋白-2比值（胰岛素样生长因子结合蛋白-2强度/总胰岛素样生长因子结合蛋白强度）显著高于正常健康绝经后女性（p < 0.02），但骨质疏松女性的血清胰岛素样生长因子结合蛋白-3比值显著低于正常健康绝经后女性（p < 0.01）。血清胰岛素样生长因子结合蛋白-2比值与腰椎骨密度（p < 0.0001）、股骨颈骨密度（p < 0.05）、大转子骨密度（p < 0.01）和Ward三角骨密度（p < 0.05）呈负相关，但血清胰岛素样生长因子结合蛋白-3比值与腰椎骨密度（p < 0.001）和大转子骨密度（p < 0.05）呈显著正相关。未发现血清胰岛素样生长因子结合蛋白水平或血清比值与骨转换生化标志物之间存在相关性。
结论
血清胰岛素样生长因子结合蛋白谱的测定可能有助于识别有骨质疏松风险的绝经后女性。
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