Louis R, Van Tulder L, Poncelet M, Corhay J L, Mendez P, Radermecker M
Department of Pneumology, CHU Sart-Timan, Liège, Belgium.
Int Arch Allergy Immunol. 1997 Mar;112(3):309-12. doi: 10.1159/000237471.
We have compared the bronchoalveolar lavage fluid (BAL) cellular composition and the BAL cell lysate histamine content (fluorometric assay) in 28 stable mild to moderate asthmatics (atopic n = 18 and intrinsic n = 10) and 11 control subjects. When compared to control subjects, the whole group of asthmatics had a higher proportion of BAL eosinophils (p < 0.01) and metachromatic cells (p < 0.05). The BAL cell lysate histamine was increased in atopic (p < 0.05) and intrinsic asthmatics (p < 0.05) in comparison with control subjects. In the whole group of asthmatics, the BAL cell lysate histamine content correlated with the percentage of BAL eosinophils (r = 0.58, p < 0.01). This relationship was significant in both atopic (r = 0.48, p < 0.05) and intrinsic (r = 0.70, p < 0.05) asthmatics. For the whole group of asthmatics, both the BAL cell lysate histamine and the percentage of BAL eosinophils inversely correlated with the percent predicted FEV1 (r = -0.42, p < 0.05; r = -0.51, p < 0.05). We conclude that an increased BAL cell lysate histamine content correlates with airway eosinophilic infiltration and lung function impairment in mild to moderate atopic and intrinsic asthmatics. This suggests that BAL mast cells play a key role in recruiting eosinophils in the airways of asthmatics irrespective of the presence of an atopic status.
我们比较了28名病情稳定的轻至中度哮喘患者（其中特应性哮喘18例，非特应性哮喘10例）和11名对照者的支气管肺泡灌洗（BAL）液细胞组成以及BAL液细胞裂解物组胺含量（荧光测定法）。与对照者相比，哮喘患者全组的BAL嗜酸性粒细胞比例更高（p<0.01），异染细胞比例更高（p<0.05）。与对照者相比，特应性哮喘患者（p<0.05）和非特应性哮喘患者（p<0.05）的BAL液细胞裂解物组胺含量均升高。在哮喘患者全组中，BAL液细胞裂解物组胺含量与BAL嗜酸性粒细胞百分比相关（r = 0.58，p<0.01）。这种关系在特应性哮喘患者（r = 0.48，p<0.05）和非特应性哮喘患者（r = 0.70，p<0.05）中均有统计学意义。对于哮喘患者全组，BAL液细胞裂解物组胺和BAL嗜酸性粒细胞百分比均与预计FEV1百分比呈负相关（r = -0.42，p<0.05；r = -0.51，p<0.05）。我们得出结论，BAL液细胞裂解物组胺含量增加与轻至中度特应性和非特应性哮喘患者的气道嗜酸性粒细胞浸润及肺功能损害相关。这表明，无论是否存在特应性状态，BAL肥大细胞在哮喘患者气道中募集嗜酸性粒细胞方面起关键作用。
