Barrat F, Lesourd B M, Louise A, Boulouis H J, Vincent-Naulleau S, Thibault D, Sanaa M, Neway T, Pilet C H
Laboratoire de Microbiologie-Immunologie et Pathologie Générale, Institut National de la Recherche Agronomique (INRA), Ecole Nationale Vétérinaire d'Alfort (ENVA), Maisons Alfort, France.
Clin Exp Immunol. 1997 Mar;107(3):593-600. doi: 10.1046/j.1365-2249.1997.3021199.x.
So far all studies on the murine ageing process have been conducted on virgin mice. Immune ageing may be influenced by sex hormone differences related to sex or pregnancies. The aim of this study was to investigate whether pregnancies and gender influence the cell changes observed during ageing in a peripheral lymphoid compartment of C57B1/6 mice. Using flow cytometry, changes in (Thy1.2+) T cell, (B220+) B cell and (CD 11b/Mac-1) macrophage spleen populations were monitored in 2, 8 (3 months after last pregnancy) 15 and 23-month-old mice including males, virgin and multiparous females. The development of naive (CD44(low)), memory (CD44(high)), activated/memory (MEL-14, CD62L) cells were investigated in CD4+ and CD8+ T cell subsets. Both short term (at 8 months) and long term (at 15 and 23 months) effects of multiparity were obvious in the lymphocyte/macrophage population changes associated with the ageing process. Short-term effects included delayed appearance of CD4+CD44(high) memory lymphocytes and increased numbers of both CD4+MEL-14(1ow) activated/memory cells and Mac-1+ macrophages when compared with virgin control mice. Later effects of multiparity were increased CD8alpha(dull) populations and increased T/B cell ratios and the ratio of memory to naive CD4+ cells (CD44+(high)/CD44+(low). A sex effect was noticed: males exhibited lower Mac-1+ levels and memory/naive ratio in CD4+ subset than virgin females throughout life. These results suggest that gender and/or pregnancies affect the age-related distribution of lymphoid and macrophage cell populations in the spleen of C57B1/6 mice.
迄今为止，所有关于小鼠衰老过程的研究都是在未交配的小鼠身上进行的。免疫衰老可能受到与性别或妊娠相关的性激素差异的影响。本研究的目的是调查妊娠和性别是否会影响C57B1/6小鼠外周淋巴区室在衰老过程中观察到的细胞变化。使用流式细胞术，监测了2、8（最后一次妊娠后3个月）、15和23月龄小鼠（包括雄性、未交配雌性和经产雌性）脾脏中（Thy1.2+）T细胞、（B220+）B细胞和（CD11b/Mac-1）巨噬细胞群体的变化。在CD4+和CD8+T细胞亚群中研究了初始（CD44（低））、记忆（CD44（高））、活化/记忆（MEL-14、CD62L）细胞的发育情况。经产在与衰老过程相关的淋巴细胞/巨噬细胞群体变化中，短期（8个月时）和长期（15和23个月时）效应均很明显。短期效应包括与未交配对照小鼠相比，CD4+CD44（高）记忆淋巴细胞出现延迟，CD4+MEL-14（低）活化/记忆细胞和Mac-1+巨噬细胞数量增加。经产的后期效应是CD8α（暗淡）群体增加，T/B细胞比率以及记忆CD4+细胞与初始CD4+细胞的比率（CD44+（高）/CD44+（低））增加。观察到了性别效应：在整个生命过程中，雄性CD4+亚群中的Mac-1+水平和记忆/初始比率均低于未交配雌性。这些结果表明，性别和/或妊娠会影响C57B1/6小鼠脾脏中淋巴细胞和巨噬细胞群体的年龄相关分布。
