Trotter K M, Wood H A
Boyce Thompson Institute at Cornell, Ithaca, NY 14853, USA.
Mol Biotechnol. 1996 Dec;6(3):329-34. doi: 10.1007/BF02761711.
The production of recombinant baculoviruses usually employs cotransfection of insect tissue-culture cells with viral and transfer-plasmid DNAs. The preparation and storage of viral and plasmid DNAs suitable for optimal transfection of insect cells are discussed. Electroporation, calcium-phosphate, and lipofection transfection techniques are presented with a discussion of their relative advantages. The rates of recombinant virus formation are compared using viral infection/plasmid transfection protocols versus cotransfection of cells with transfer-plasmid and viral DNAs.
重组杆状病毒的生产通常采用将病毒DNA和转移质粒DNA共转染昆虫组织培养细胞的方法。本文讨论了适合昆虫细胞最佳转染的病毒DNA和质粒DNA的制备及储存。介绍了电穿孔、磷酸钙和脂质体转染技术，并讨论了它们的相对优势。比较了使用病毒感染/质粒转染方案与细胞用转移质粒和病毒DNA共转染时重组病毒形成的速率。
