• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脉冲场梯度电泳中大型环状DNA“异常”迁移率的表征

Characterization of the 'unusual' mobility of large circular DNAs in pulsed field-gradient electrophoresis.

作者信息

Beverley S M

机构信息

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115.

出版信息

Nucleic Acids Res. 1988 Feb 11;16(3):925-39. doi: 10.1093/nar/16.3.925.

DOI:10.1093/nar/16.3.925
PMID:3344223
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334728/
Abstract

Large circular amplified DNAs (30 and 85 kb) present in methotrexate-resistant Leishmania major appear to migrate anomalously in pulsed field-gradient electrophoresis (PFGE), exhibiting pulse time-dependent mobility and migrating along a different apparent path relative to the large linear chromosomal DNAs. Quantitative studies indicate that the relative pulse-time dependence is actually conferred by the mobility properties of the large linear DNAs. One contributing factor to the difference in migration path is variability in the intrinsic voltage-dependence of mobility of supercoiled and linear DNAs, in combination with the asymmetrical/inhomogeneous voltage gradients. Certain linear chromosomes exhibit a previously undescribed pulse-time dependence in the voltage-dependence of mobility. When enzymatically relaxed or physically nicked the large circular DNAs fail to leave the well using any pulse time, a property also observed in conventional electrophoresis. These findings are relevant to PFGE theory, and its application to the study of circular DNA amplification in Leishmania and other species.

摘要

存在于耐甲氨蝶呤的硕大利什曼原虫中的大型环状扩增DNA（30和85 kb）在脉冲场梯度电泳（PFGE）中似乎迁移异常，表现出脉冲时间依赖性迁移率，并且相对于大型线性染色体DNA沿着不同的表观路径迁移。定量研究表明，相对脉冲时间依赖性实际上是由大型线性DNA的迁移率特性赋予的。迁移路径差异的一个促成因素是超螺旋和线性DNA迁移率的内在电压依赖性的变异性，以及不对称/不均匀的电压梯度。某些线性染色体在迁移率的电压依赖性方面表现出以前未描述的脉冲时间依赖性。当通过酶促松弛或物理切口处理时，大型环状DNA在任何脉冲时间都无法离开加样孔，这也是在传统电泳中观察到的特性。这些发现与PFGE理论及其在利什曼原虫和其他物种中环状DNA扩增研究中的应用相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2816/334728/7b7ac19ea524/nar00145-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2816/334728/49059037b33c/nar00145-0146-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2816/334728/88049e1e8f8e/nar00145-0147-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2816/334728/b62f30142975/nar00145-0148-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2816/334728/120c4877f682/nar00145-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2816/334728/7b7ac19ea524/nar00145-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2816/334728/49059037b33c/nar00145-0146-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2816/334728/88049e1e8f8e/nar00145-0147-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2816/334728/b62f30142975/nar00145-0148-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2816/334728/120c4877f682/nar00145-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2816/334728/7b7ac19ea524/nar00145-0152-a.jpg

相似文献

1
Characterization of the 'unusual' mobility of large circular DNAs in pulsed field-gradient electrophoresis.脉冲场梯度电泳中大型环状DNA“异常”迁移率的表征
Nucleic Acids Res. 1988 Feb 11;16(3):925-39. doi: 10.1093/nar/16.3.925.
2
Migration properties of circular DNAs using orthogonal-field-alternation gel electrophoresis.使用正交交变电场凝胶电泳分析环状DNA的迁移特性
Electrophoresis. 1989 May-Jun;10(5-6):283-90. doi: 10.1002/elps.1150100503.
3
Analysis of amplified DNAs from drug-resistant Leishmania by orthogonal-field-alternation gel electrophoresis. The effect of size and topology on mobility.通过正交交变电场凝胶电泳分析耐药利什曼原虫的扩增DNA。大小和拓扑结构对迁移率的影响。
J Biol Chem. 1989 Feb 15;264(5):2979-84.
4
High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 4. Influence of DNA topology.DNA脉冲场凝胶电泳中的高分辨率分离及精确大小测定。4. DNA拓扑结构的影响。
Biochemistry. 1988 Dec 27;27(26):9222-6. doi: 10.1021/bi00426a022.
5
Pulsed-field gel electrophoresis of circular DNA.环状DNA的脉冲场凝胶电泳
Nucleic Acids Res. 1989 Jun 12;17(11):4359-65. doi: 10.1093/nar/17.11.4359.
6
Pulsed field separation of large supercoiled and open-circular DNAs and its application to bacterial artificial chromosome cloning.大型超螺旋和开环DNA的脉冲场分离及其在细菌人工染色体克隆中的应用。
Electrophoresis. 1995 Jan;16(1):1-7. doi: 10.1002/elps.1150160102.
7
Pulse time and agarose concentration affect the electrophoretic mobility of cccDNA during PFGE and FIGE [corrected].脉冲时间和琼脂糖浓度会影响脉冲场凝胶电泳（PFGE）和交变脉冲电场凝胶电泳（FIGE）过程中cccDNA的电泳迁移率[已修正]。
Nucleic Acids Res. 1989 Sep 25;17(18):7359-69. doi: 10.1093/nar/17.18.7359.
8
Estimation of circular DNA size using gamma-irradiation and pulsed-field gel electrophoresis.
Anal Biochem. 1989 Feb 15;177(1):110-4. doi: 10.1016/0003-2697(89)90023-7.
9
Amplified DNAs in laboratory stocks of Leishmania tarentolae: extrachromosomal circles structurally and functionally similar to the inverted-H-region amplification of methotrexate-resistant Leishmania major.实验室保藏的热带利什曼原虫中的扩增DNA：染色体外环状结构，在结构和功能上与耐甲氨蝶呤的硕大利什曼原虫的倒H区扩增相似。
Mol Cell Biol. 1988 Dec;8(12):5188-99. doi: 10.1128/mcb.8.12.5188-5199.1988.
10
Separation of closed circular DNA from linear DNA by electrophoresis in two dimensions in agarose gels.在琼脂糖凝胶中通过二维电泳从线性DNA中分离闭环DNA。
Nucleic Acids Res. 1981 Dec 21;9(24):6805-12. doi: 10.1093/nar/9.24.6805.

引用本文的文献

1
Characterization of Temperate LPS-Binding Bordetella avium Phages That Lack Superinfection Immunity.温和型 LPS 结合鲍氏不动杆菌噬菌体的特性研究，这些噬菌体缺乏超感染免疫性。
Microbiol Spectr. 2023 Jun 15;11(3):e0370222. doi: 10.1128/spectrum.03702-22. Epub 2023 May 1.
2
Quantitative assessment reveals the dominance of duplicated sequences in germline-derived extrachromosomal circular DNA.定量评估揭示了生殖系衍生的染色体外环状 DNA 中重复序列的优势。
Proc Natl Acad Sci U S A. 2021 Nov 23;118(47). doi: 10.1073/pnas.2102842118.
3
Extrachromosomal DNA amplicons in antimalarial-resistant Plasmodium falciparum.

本文引用的文献

1
A comparative study of different Leishmania tropica isolates from Iran: correlation between infectivity and cytochemical properties.伊朗不同热带利什曼原虫分离株的比较研究：感染性与细胞化学特性之间的相关性。
Am J Trop Med Hyg. 1983 Jul;32(4):694-702. doi: 10.4269/ajtmh.1983.32.694.
2
Overproduction of a bifunctional thymidylate synthetase-dihydrofolate reductase and DNA amplification in methotrexate-resistant Leishmania tropica.
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2132-6. doi: 10.1073/pnas.80.8.2132.
3
Unstable DNA amplifications in methotrexate-resistant Leishmania consist of extrachromosomal circles which relocalize during stabilization.甲氨蝶呤抗性利什曼原虫中的不稳定DNA扩增由染色体外环状结构组成，这些环状结构在稳定过程中重新定位。
疟原虫抗药性中外源 DNA 扩增子。
Mol Microbiol. 2021 Apr;115(4):574-590. doi: 10.1111/mmi.14624. Epub 2020 Nov 19.
4
Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.DNA重组与修复研究指南：DNA修复途径的细胞分析
Microb Cell. 2019 Jan 7;6(1):1-64. doi: 10.15698/mic2019.01.664.
5
Degradation of RNA during lysis of Escherichia coli cells in agarose plugs breaks the chromosome.在琼脂糖凝胶块中裂解大肠杆菌细胞时RNA的降解会破坏染色体。
PLoS One. 2017 Dec 21;12(12):e0190177. doi: 10.1371/journal.pone.0190177. eCollection 2017.
6
Pulsed-field gel electrophoresis does not break E. coli chromosome undergoing excision repair after UV irradiation.脉冲场凝胶电泳不会打断紫外线照射后正在进行切除修复的大肠杆菌染色体。
Anal Biochem. 2017 Jun 1;526:66-68. doi: 10.1016/j.ab.2017.03.019. Epub 2017 Mar 27.
7
Differential Gene Expression and Infection Profiles of Cutaneous and Mucosal Leishmania braziliensis Isolates from the Same Patient.来自同一患者的皮肤型和黏膜型巴西利什曼原虫分离株的差异基因表达及感染特征
PLoS Negl Trop Dis. 2015 Sep 14;9(9):e0004018. doi: 10.1371/journal.pntd.0004018. eCollection 2015.
8
Macrotene chromosomes provide insights to a new mechanism of high-order gene amplification in eukaryotes.粗线期染色体为真核生物高阶基因扩增的新机制提供了见解。
Nat Commun. 2015 Jan 30;6:6154. doi: 10.1038/ncomms7154.
9
Molecular characterization of the MRPA transporter and antimony uptake in four New World Leishmania spp. susceptible and resistant to antimony.四种易感性和抗锑性新世界利什曼原虫中 MRPA 转运蛋白的分子特征和锑摄取。
Int J Parasitol Drugs Drug Resist. 2013 Sep 5;3:143-53. doi: 10.1016/j.ijpddr.2013.08.001. eCollection 2013 Dec.
10
Trapping and breaking of in vivo nicked DNA during pulsed field gel electrophoresis.在脉冲场凝胶电泳中体内切口 DNA 的捕获和断裂。
Anal Biochem. 2013 Dec 15;443(2):269-81. doi: 10.1016/j.ab.2013.06.001. Epub 2013 Jun 14.
Cell. 1984 Sep;38(2):431-9. doi: 10.1016/0092-8674(84)90498-7.
4
Gene amplification.基因扩增
Annu Rev Biochem. 1984;53:447-91. doi: 10.1146/annurev.bi.53.070184.002311.
5
Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.通过正交交变电场凝胶电泳从酵母中分离染色体DNA分子。
Nucleic Acids Res. 1984 Jul 25;12(14):5647-64. doi: 10.1093/nar/12.14.5647.
6
Gene amplification in cultured animal cells.培养动物细胞中的基因扩增。
Cell. 1984 Jul;37(3):705-13. doi: 10.1016/0092-8674(84)90406-9.
7
Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.通过脉冲场梯度凝胶电泳分离酵母染色体大小的DNA。
Cell. 1984 May;37(1):67-75. doi: 10.1016/0092-8674(84)90301-5.
8
Conformation of double-stranded DNA during agarose gel electrophoresis: fractionation of linear and circular molecules with molecular weights between 3 X 10(6) and 26 X 10(6).琼脂糖凝胶电泳过程中双链DNA的构象：分子量在3×10⁶至26×10⁶之间的线性和环状分子的分离
Biochemistry. 1984 Feb 28;23(5):922-7. doi: 10.1021/bi00300a020.
9
Selective trapping of circular double-stranded DNA molecules in solidifying agarose.
Plasmid. 1982 May;7(3):263-70. doi: 10.1016/0147-619x(82)90007-5.
10
DNA topoisomerases.DNA拓扑异构酶
Annu Rev Biochem. 1981;50:879-910. doi: 10.1146/annurev.bi.50.070181.004311.