交联蛋白的完全酶促水解

The complete enzymic hydrolysis of crosslinked proteins.

作者信息

Milligan B, Holt L A

出版信息

Adv Exp Med Biol. 1977;86B:277-84. doi: 10.1007/978-1-4757-9113-6_19.

Abstract

Procedures used for the complete enzymic hydrolysis of proteins are reviewed. The successful application of complete enzymic hydrolysis in the detection of naturally occurring isopeptide crosslinks and various other types of chemically introduced crosslinks is described. The method may fail if the level of crosslinking is too high, or if crosslinking is accompanied by racemization. Although it is usual to cleave disulphide crosslinks prior to enzymic digestion, the necessity for this in all cases is questioned.

摘要

本文综述了用于蛋白质完全酶解的方法。描述了完全酶解在天然存在的异肽交联和各种其他化学引入交联检测中的成功应用。如果交联水平过高，或者交联伴有消旋作用，该方法可能会失败。虽然通常在酶解之前切断二硫键交联，但对所有情况下这样做的必要性提出了质疑。