Feng Y, Gregor P
Institute for Metabolic Disorders, Bayer Corporation, West Haven, Connecticut 06516, USA.
Biochem Biophys Res Commun. 1997 Feb 24;231(3):651-4. doi: 10.1006/bbrc.1997.6161.
We have used PCR with degenerate oligonucleotide primers to clone novel members of the G protein-coupled receptor (GPCR) superfamily. We report here a novel gene, CEPR, which encodes a candidate receptor that is most similar to the peptide receptor family. The coding region of the human CEPR gene predicts a seven transmembrane domain (TM) receptor of 375 amino acids. CEPR has 28-30 percent amino acid identity to angiotensin II and interleukin 8 receptors, and slightly lower percent identity to many other GPCRs. Northern blot analysis reveals a 3.3 kb CEPR transcript in different regions of human brain and in various peripheral tissues. The ubiquitous tissue distribution of CEPR, its expression in early development, and its conservation in evolution indicate a potentially important biological function for this receptor and its putative peptide ligand.
我们利用简并寡核苷酸引物进行聚合酶链反应（PCR），以克隆G蛋白偶联受体（GPCR）超家族的新成员。我们在此报告一个新基因CEPR，它编码一种与肽受体家族最为相似的候选受体。人类CEPR基因的编码区预测有一个由375个氨基酸组成的七跨膜结构域（TM）受体。CEPR与血管紧张素II和白细胞介素8受体有28%-30%的氨基酸同一性，与许多其他GPCR的同一性略低。Northern印迹分析显示，在人类大脑的不同区域和各种外周组织中存在一个3.3 kb的CEPR转录本。CEPR在组织中的广泛分布、其在早期发育中的表达以及在进化中的保守性表明，该受体及其假定的肽配体具有潜在的重要生物学功能。
