Matsuda Y, Saito T, Fujimoto J, Yamamoto T
Genome Research Group, National Institute of Radiological Sciences, Chiba, Japan.
Genomics. 1997 Feb 15;40(1):181-4. doi: 10.1006/geno.1996.4553.
brt encodes a receptor-type protein-tyrosine kinase and was isolated from fetal mouse brain by using a PCR-mediated cloning procedure; this gene is expressed preferentially in the brain of both embryo and adult. Chromosomal locations of the mouse and rat brt genes were determined by fluorescence in situ hybridization using a mouse cDNA fragment as a probe. The brt gene was localized to mouse Chromosome 2F1 and rat Chromosome 3q36.1, where a conserved linkage homology has been identified between the two species. Interspecific backcross analysis genetically demonstrated no recombination between the mouse BRT locus and D2Mit63.
brt编码一种受体型蛋白酪氨酸激酶，通过PCR介导的克隆程序从小鼠胚胎脑分离得到；该基因在胚胎和成年小鼠的脑中均优先表达。使用小鼠cDNA片段作为探针，通过荧光原位杂交确定了小鼠和大鼠brt基因的染色体定位。brt基因定位于小鼠2F1染色体和大鼠3q36.1染色体，在这两个物种之间已鉴定出保守的连锁同源性。种间回交分析从遗传学上证明小鼠BRT基因座与D2Mit63之间没有重组。
