Kawamura K
Department of Anesthesiology, Tottori University Hospital, Yonago.
Masui. 1997 Feb;46(2):222-8.
While various additive solutions, containers and temperature have been shown to improve qualities of stored red cells, there are no reports on gas environment during blood storage. Nitrous oxide (N2O) showed a very high permeability through polyvinyl chloride membrane and high blood solubility. The whole blood with CPD was stored in N2 O (N2O-group) and compared with the control which was stored in air (Air-group). After storage (21 days at 5 degrees C and 35 days at 1 degree C), N2O-group showed significantly lower decreases of pH as well as significantly lower increases of Pco2 than Air-group. At both temperatures, glucose concentrations and ATP levels showed no differences between the two groups, but concentrations of lactate and 2, 3-DPG in N2O-group are significantly higher than those in Air-group. It is concluded that nitrous oxide gas environment can prevent decrease of pH by the suppression of increasing Pco2 in the stored blood due to high permeability and solubility into the cooled blood from ambient and can maintain functions of stored red cells, while neither oxidation toxicity nor antioxidation effects of N2O were demonstrated.
虽然各种添加剂溶液、容器和温度已被证明可改善储存红细胞的质量，但关于血液储存期间的气体环境尚无相关报道。氧化亚氮（N2O）对聚氯乙烯膜具有极高的渗透性且在血液中的溶解度很高。将添加枸橼酸盐-磷酸盐-葡萄糖（CPD）的全血储存在N2O中（N2O组），并与储存在空气中的对照组（空气组）进行比较。储存后（5℃下储存21天，1℃下储存35天），N2O组的pH值下降幅度显著低于空气组，Pco2的升高幅度也显著低于空气组。在这两个温度下，两组的葡萄糖浓度和ATP水平均无差异，但N2O组的乳酸盐和2,3-二磷酸甘油酸（2,3-DPG）浓度显著高于空气组。得出的结论是，氧化亚氮气体环境可通过抑制因环境中氧化亚氮具有高渗透性和高溶解度而进入冷藏血液中导致储存血液中Pco2升高来防止pH值下降，并可维持储存红细胞的功能，而氧化亚氮既未表现出氧化毒性也未表现出抗氧化作用。
