Borderie V M, Baudrimont M, Lopez M, Carvajal S, Laroche L
Service d'Ophtalmologie, Hôpital Saint Antoine, Paris, France.
Cornea. 1997 Mar;16(2):215-23.
To evaluate corneal structural modifications induced by the deswelling period in dextran-containing medium following organ culture.
Twenty human corneas were organ-cultured for 2 weeks and subsequently incubated in Exosol deswelling medium (Opsia, Toulouse, France) for 1-4 days. Corneas were studied by means of light microscopy, morphometry, and transmission electron microscopy.
The deswelling period induced a statistically significant 8.4% endothelial cell loss and a 27.4% increase in the coefficient of variation. The endothelial layer remained intact. The basal epithelial cells displayed a flat appearance. Thin endothelial cells were observed in addition to dark vacuoles (the number of which increased with incubation time) with dense material and no mitochondrial swelling. Some basal epithelial cells and keratocytes were damaged on day 3 or 4.
Preservation injuries induced by 1 or 2 days of incubation in deswelling medium are moderate. Three or 4 days of incubation result in more severe dextran-induced injuries. Consequently, the deswelling period should not exceed 2 days.
评估器官培养后在含右旋糖酐培养基中消肿期诱导的角膜结构改变。
将20个人类角膜进行器官培养2周，随后在Exosol消肿培养基（法国图卢兹的Opsia公司）中孵育1 - 4天。通过光学显微镜、形态测量学和透射电子显微镜对角膜进行研究。
消肿期导致内皮细胞有统计学意义的8.4%损失以及变异系数增加27.4%。内皮层保持完整。基底上皮细胞呈现扁平外观。除了暗泡（其数量随孵育时间增加）、致密物质和无线粒体肿胀外，还观察到薄的内皮细胞。在第3天或第4天，一些基底上皮细胞和角膜细胞受损。
在消肿培养基中孵育1或2天引起的保存损伤是中度的。孵育3或4天会导致更严重的右旋糖酐诱导的损伤。因此，消肿期不应超过2天。
