Hamasaki-Katagiri N, Tabor C W, Tabor H
Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0830, USA.
Gene. 1997 Mar 10;187(1):35-43. doi: 10.1016/s0378-1119(96)00660-9.
The Saccharomyces cerevisiae SPE3 gene, coding for spermidine synthase, was cloned, sequenced, and localized on the right arm of chromosome XVI. The deduced amino acid sequence has a high similarity to mammalian spermidine synthases, and has putative S-adenosylmethionine binding motifs. To investigate the effect of total loss of the SPE3 gene, we constructed a null mutant of this gene, spe3delta, which has no spermidine synthase activity and has an absolute requirement for spermidine or spermine for the growth. This requirement is satisfied by a very low concentration of spermidine (10(-8) M) or a higher concentration of spermine (10(-6) M).
编码亚精胺合酶的酿酒酵母SPE3基因被克隆、测序并定位在第十六条染色体的右臂上。推导的氨基酸序列与哺乳动物亚精胺合酶具有高度相似性，并且具有假定的S-腺苷甲硫氨酸结合基序。为了研究SPE3基因完全缺失的影响，我们构建了该基因的缺失突变体spe3delta，其没有亚精胺合酶活性，并且生长绝对需要亚精胺或精胺。极低浓度的亚精胺（10^(-8) M）或较高浓度的精胺（10^(-6) M）可以满足这一需求。
