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从植物乳杆菌中分离、提取和测定乙酰胆碱。

Isolation, extraction, and measurement of acetylcholine from Lactobacillus plantarum.

作者信息

Stanaszek P M, Snell J F, O'Neill J J

出版信息

Appl Environ Microbiol. 1977 Aug;34(2):237-9. doi: 10.1128/aem.34.2.237-239.1977.

DOI:10.1128/aem.34.2.237-239.1977
PMID:907345
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC242629/
Abstract

The isolation, extraction, and spectrophotometric determination of acetylcholine from Lactobacillus plantarum ATCC 10241 is described. Acetylcholine was extracted with a mixture of sodium tetraphenylboron-butylethylketone-acetonitrile and was measured enzymatically at 340 nm.

摘要

描述了从植物乳杆菌ATCC 10241中分离、提取和分光光度法测定乙酰胆碱的方法。用四苯基硼酸钠-丁基乙基酮-乙腈混合物提取乙酰胆碱，并在340nm处进行酶促测定。

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Isolation, extraction, and measurement of acetylcholine from Lactobacillus plantarum.从植物乳杆菌中分离、提取和测定乙酰胆碱。
Appl Environ Microbiol. 1977 Aug;34(2):237-9. doi: 10.1128/aem.34.2.237-239.1977.
2
[A sensitive determination of choline and acetylcholine by tetraphenylboron sodium].[用四苯硼钠灵敏测定胆碱和乙酰胆碱]
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3
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A comparison of chemiluminescent and radioenzymatic methods for the measurement of acetylcholine released from a rat phrenic nerve-hemidiaphragm preparation.化学发光法与放射酶法用于测量大鼠膈神经 - 半膈肌标本释放乙酰胆碱的比较。
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6
[Bioassay for acetylcholine from the rat cervical sympathetic ganglion (author's transl)].大鼠颈交感神经节乙酰胆碱的生物测定（作者译）
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Isolation of choline and choline esters from Krebs-Ringer solution for gas chromatographic determination.从克雷布斯-林格溶液中分离胆碱和胆碱酯用于气相色谱测定。
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Sensitive spectrophotometric determination of tetraphenylboron and tetraphenylarsonium with ethidium bromide.
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本文引用的文献

1
Studies of the acetyl coenzyme A synthetase reaction. II. Crystalline acetyl coenzyme A synthetase.乙酰辅酶A合成酶反应的研究。II. 结晶乙酰辅酶A合成酶。
J Biol Chem. 1965 Nov;240(11):4158-63.
2
Isolation of choline esters from aqueous solutions by extraction with sodium tetraphenylboron in organic solvents.通过在有机溶剂中用四苯基硼酸钠萃取从水溶液中分离胆碱酯。
Biochem J. 1969 Jun;113(2):291-8. doi: 10.1042/bj1130291.
3
Identification of acetylcholine in fresh rat brain by combined gas chromatography-mass spectrometry.采用气相色谱-质谱联用技术鉴定新鲜大鼠脑中的乙酰胆碱。
Nature. 1968 Nov 30;220(5170):915-7. doi: 10.1038/220915a0.
4
Extraction of acetylcholine from aqueous solutions.从水溶液中提取乙酰胆碱。
Biochem Pharmacol. 1968 Dec;17(12):2503-5. doi: 10.1016/0006-2952(68)90146-9.
5
Enzymatic fluorometric determination of acetylcholine in biological extracts.
J Neurochem. 1970 Oct;17(10):1451-60. doi: 10.1111/j.1471-4159.1970.tb00512.x.