来自日本金龟子芽孢杆菌的假定滚环质粒pBP614的特性分析。

Characterization of pBP614, a putative rolling-circle plasmid from Bacillus popilliae.

作者信息

Longley M, MacDonald R, Poulter T M

机构信息

Department of Biochemistry, University of Otago, Dunedin, New Zealand.

出版信息

Plasmid. 1997;37(1):15-21. doi: 10.1006/plas.1996.1275.

DOI:10.1006/plas.1996.1275

PMID:9073578

Abstract

A plasmid, pBP614 (5.647 kb), has been isolated from the New Zealand Bacillus popilliae strain 17 and characterized by physical mapping, cloning, and sequencing. An open reading frame was found which could encode a protein with homology to the replication (Rep) proteins of rolling-circle plasmids. The predicted B. popilliae Rep protein shows closest homology to Rep proteins from Bacillus plasmids of the pC194 family. Sequences homologous to plus- and minus-strand replication origins were found. The minus-strand origin shows similarities to the pal-T type family. Taken together, these observations suggest that pBP614 is a rolling-circle plasmid, the first such plasmid characterized from B. popilliae.

摘要

从新西兰绿僵菌芽孢杆菌菌株17中分离出了一种质粒pBP614（5.647 kb），并通过物理图谱绘制、克隆和测序对其进行了表征。发现了一个开放阅读框，它可以编码一种与滚环质粒的复制（Rep）蛋白具有同源性的蛋白质。预测的绿僵菌芽孢杆菌Rep蛋白与pC194家族芽孢杆菌质粒的Rep蛋白具有最密切的同源性。发现了与正链和负链复制起点同源的序列。负链起点与pal-T型家族相似。综合这些观察结果表明，pBP614是一种滚环质粒，是首个从绿僵菌芽孢杆菌中鉴定出的此类质粒。